| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Muellerian-inhibiting factor;Anti-Muellerian hormone;AMH;Muellerian-inhibiting substance;MIS;AMH;MIF; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived rat Ccl21 recombinant protein (Position: S24-Q133). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-Ccl21 Antibody Picoband® is an antibody for Ccl21 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: Ccl21 (Muellerian-inhibiting factor); UniProt: Q5RJN3
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 15 kDa, calculated 59195 MW
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-Ccl21 Antibody Picoband® catalog # A00765-2.
Biological background
Biological context: This glycoprotein, produced by the Sertoli cells of the testis, causes regression of the Muellerian duct. It is also able to inhibit the growth of tumors derived from tissues of Muellerian duct origin.
Expression and localization notes: cellular localization: Secreted., tissue context: Predominantly expressed in lung and at lower level in kidney. Expressed in macrophages but not in vascular smooth muscle cells..
Common research applications
- Western blotting (WB): Compare Ccl21 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of Ccl21 in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify Ccl21-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: Chemokine (C-C motif) ligand 21 (CCL21) is a small cytokine belonging to the CC chemokine family. This antimicrobial gene is one of several CC cytokine genes clustered on the p-arm of chromosome 9. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. Similar to other chemokines the protein encoded by this gene inhibits hemopoiesis and stimulates chemotaxis. This protein is chemotactic in vitro for thymocytes and activated T cells, but not for B cells, macrophages, or neutrophils. The cytokine encoded by this gene may also play a role in mediating homing of lymphocytes to secondary lymphoid organs. It is a high affinity functional ligand for chemokine receptor 7 that is expressed on T and B lymphocytes and a known receptor for another member of the cytokine family (small inducible cytokine A19).
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Secreted.
- Tissue details: Predominantly expressed in lung and at lower level in kidney. Expressed in macrophages but not in vascular smooth muscle cells.
- Research category: Developmental Biology,Growth Factors/Hormones,Reproduction,Secreted,Signal Transduction,Signaling Pathways,Stem Cells,TGF Beta
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
