Anti-CCL4/MIP1 beta Rabbit Monoclonal Antibody

Overview

This product is an anti-CCL4 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone 23C55; isotype IgG; reactivity: Human,Mouse. Reported application contexts include WB, ICC, IF, IP (as provided in the source record). Boster Bio Anti-CCL4/MIP1 beta Rabbit Monoclonal Antibody catalog # M00703. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human, Mouse.

Key elements and design rationale

• Target: CCL4 (Cyclin-A2).
• Antibody format: Monoclonal; clone 23C55; isotype IgG.
• Host: Rabbit.
• Species reactivity: Human,Mouse (confirm in your model system with appropriate controls).

This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.

Biological background

CCL4 (protein: T-cell surface glycoprotein CD4) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Essential for the control of the cell cycle at the G1/S (start) and the G2/M (mitosis) transitions. Reported cellular localization context: Nucleus . Cytoplasm . Cytoplasmic when associated with SCAPER. Tissue expression notes (as provided): Expressed in striatal neurons of patients with Huntington disease (at protein level). Brain, kidney, placenta, colon, heart, liver, spleen, skeletal muscle, prostate, thymus and pancreas. Highly expressed in fetal tissue. .

Research relevance and current trends

• Research context keywords from the source record include: Cancer,Cardiovascular,Cell Biology,Cell Cycle,Epigenetics and Nuclear Signaling,Lipids/Lipoproteins.
• Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
• Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.

Common research applications

• Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
• Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
• Immunoprecipitation (IP): enrich target complexes for downstream immunoblot or interaction analyses.

Workflow ideas (metafield): Validate CCL4 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect CCL4 expression by Western blot in cell or tissue lysates, Localize CCL4 by immunofluorescence/immunocytochemistry in cultured cells, Enrich CCL4 by immunoprecipitation from lysates for downstream analysis

Notes for experimental interpretation

• Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
• Apparent molecular weight may vary by sample type and processing (observed MW: 11 kDa; calculated MW: 48551 MW).
• Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.

Additional product details (from the source record)

• Molecular weight (observed): 11 kDa
• Cellular localization (provided): Nucleus . Cytoplasm . Cytoplasmic when associated with SCAPER.
• Tissue details (provided): Expressed in striatal neurons of patients with Huntington disease (at protein level). Brain, kidney, placenta, colon, heart, liver, spleen, skeletal muscle, prostate, thymus and pancreas. Highly expressed in fetal tissue. .

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.