| Field | Specification |
|---|---|
| Target | |
| Alternative names | C-C motif chemokine 6; Protein C10; Small-inducible cytokine A6; CCL6 (22-95); CCL6 (23-95); Ccl6; C10; Scya6 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived mouse CCL6 recombinant protein (Position: G22-A116). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects C-C motif chemokine 6 (gene Ccl6) in mouse and rat samples and is validated for ELISA, IHC and Western blot. The predicted molecular weight is 13.0 kDa, and the supplier reports an observed band at about 11 kDa.
In mouse, CCL6 (also called C10) is a CC chemokine with no direct human counterpart. It mainly attracts macrophages and can also recruit B cells, CD4+ T cells and eosinophils, so it is used as a marker of myeloid cell recruitment in mouse models of inflammation and tissue injury.
| Target | C-C motif chemokine 6 (gene Ccl6; UniProt P27784, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 13.0 kDa |
| Observed MW | 11 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL |
| ELISA (capture antibody) | 1–5 µg/mL |
Conditions in the example images (WB): 5–20% gradient SDS-PAGE, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): mouse spleen tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Immunogen
Recombinant mouse CCL6 fragment (Gly22–Ala116), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt describes C-C motif chemokine 6 as a secreted protein. Tissue expression noted by UniProt (mouse): Expressed in myelopoietic bone marrow cultures stimulated by GM-CSF. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yumeng Li et al. (2024) Exploring the mechanism of Taohong Siwu Decoction in treating ischemic stroke injury via the circDnajc1/miR-27a-5p/C1qc signaling axis. Phytomedicine. 10.1016/j.phymed.2024.156305
