| Field | Specification |
|---|---|
| Target | |
| Alternative names | C-C chemokine receptor type 1;C-C CKR-1;CC-CKR-1;CCR-1;CCR1;Macrophage inflammatory protein 1-alpha receptor;MIP-1alpha-R;RANTES-R;CD191;Ccr1;Cmkbr1; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived mouse CCR1 recombinant protein (Position: A168-T339). Mouse CCR1 shares 76.5% amino acid (aa) sequence identity with human CCR1. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets C-C chemokine receptor type 1 (gene Ccr1) in mouse and rat samples and is validated for Western blot. The predicted molecular weight is 40.9 kDa, and the supplier reports an observed band at about 48 kDa.
CCR1 is a G protein-coupled chemokine receptor that binds several CC chemokines, including CCL3, CCL5 and CCL7. It guides monocytes, macrophages, T cells and dendritic cells to sites of inflammation, and it is studied in models of inflammatory disease, transplant rejection and bone remodeling.
| Target | C-C chemokine receptor type 1 (gene Ccr1; UniProt P51675, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 40.9 kDa |
| Observed MW | 48 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Rat, Mouse) |
|---|
Samples with a confirmed band (WB): rat PC-12 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Immunogen
Recombinant mouse CCR1 fragment (Ala168–Thr339), expressed in E. coli. Sequence identity with the human ortholog: 76.5%.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt places C-C chemokine receptor type 1 in the cell membrane. Tissue expression noted by UniProt (mouse): Detected in the heart, spleen, lung, peritoneal exudate cells and leukocytes. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Mei Huang et al. (2016) A novel multi-target RNAi adenovirus inhibits hepatoma cell proliferation, migration, and induction of angiogenesis. Oncotarget. 10.18632/oncotarget.9531
Tomasz Radzik et al. (2019) Calcium Dyshomeostasis Alters CCL5 Signaling in Differentiated PC12 Cells. Biomed Research International. 10.1155/2019/9616248
