| Field | Specification |
|---|---|
| Target | |
| Alternative names | C-C chemokine receptor type 2; C-C CKR-2; CC-CKR-2; CC-CKR-2; CCR-2; CCR2; JE/FIC receptor; MCP-1 receptor; CD192; Ccr2; Cmkbr2 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of mouse CCR2, which shares 85.7% and 95.5% amino acid (aa) sequence identity with human and rat CCR2, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets C-C chemokine receptor type 2 (gene Ccr2) in mouse and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 42.8 kDa, and the supplier reports an observed band at about 45 kDa.
CCR2 is the main receptor for the chemokine CCL2 (MCP-1) and also responds to CCL7 and related chemokines. It drives the exit of inflammatory monocytes from the bone marrow and their migration into tissues, and it is widely studied in atherosclerosis, obesity-related inflammation, neuroinflammation and cancer.
| Target | C-C chemokine receptor type 2 (gene Ccr2; UniProt P51683, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 42.8 kDa |
| Observed MW | 45 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Mouse, Rat) |
Samples with a confirmed band (WB): rat brain tissue, rat lung tissue, mouse brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse spleen tissue, rat spleen tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide from the C-terminal region of mouse CCR2. Sequence identity with the human and rat orthologs: 85.7% and 95.5%, respectively.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt places C-C chemokine receptor type 2 in the cell membrane. Tissue expression noted by UniProt (mouse): Epressed in mature thymocytes. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Hanlin Shi et al. (2024) RNA-seq combined network pharmacology reveals that Fu-Gan-Wan (FGW) inhibits liver fibrosis via NF-κB/CCL2/CCR2 and lipid peroxidation via Nrf2/HMOX1 signaling pathway. Journal of Ethnopharmacology. 10.1016/j.jep.2024.117963
Xiang Xie et al. (2026) Aerobic Exercise Ameliorates Adverse Vascular Remodeling in Diabetes via PDK1/FoxO1 Axis. FASEB Journal. 10.1096/fj.202504631R
Xianghong Lei et al. (2020) Micheliolide Attenuates Lipopolysaccharide-Induced Inflammation by Modulating the mROS/NF-κB/NLRP3 Axis in Renal Tubular Epithelial Cells. Mediators of Inflammation. 10.1155/2020/3934769
Yungong Wang et al. (2018) Nuclear factor kappa B regulated monocyte chemoattractant protein-1/chemokine CC motif receptor-2 expressing in spinal cord contributes to the maintenance of cancer-induced bone pain in rats. Molecular Pain. 10.1177/1744806918788681
