| Field | Specification |
|---|---|
| Target | |
| Alternative names | C-C chemokine receptor type 3;C-C CKR-3;CC-CKR-3;CCR-3;CCR3;CKR3;Eosinophil eotaxin receptor;CD193;CCR3;CMKBR3; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human CCR3 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ECE-3 is a rabbit monoclonal antibody that recognizes C-C chemokine receptor type 3 (gene CCR3) in human, mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 41.0 kDa, and the supplier reports an observed band at about 50 kDa.
CCR3 is a G protein-coupled receptor for eosinophil-attracting chemokines such as eotaxin (CCL11), eotaxin-2 and eotaxin-3. It is highly expressed on eosinophils, basophils and some Th2 cells, and it is a key molecule in studies of allergic inflammation and asthma.
| Target | C-C chemokine receptor type 3 (gene CCR3; UniProt P51677, human) |
|---|---|
| Clone | ECE-3 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 41.0 kDa |
| Observed MW | 50 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): human K562 cells, human U87 cells, rat brain tissue, rat C6 cells, mouse brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human epidemal cells of skin tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human CCR3.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places C-C chemokine receptor type 3 in the cell membrane. Tissue expression noted by UniProt (human): In eosinophils as well as trace amounts in neutrophils and monocytes. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Tomasz Radzik et al. (2019) Calcium Dyshomeostasis Alters CCL5 Signaling in Differentiated PC12 Cells. Biomed Research International. 10.1155/2019/9616248
