| Field | Specification |
|---|---|
| Target | |
| Alternative names | C-C chemokine receptor type 7;C-C CKR-7;CC-CKR-7;CCR-7;BLR2;CDw197;Epstein-Barr virus-induced G-protein coupled receptor 1;EBI1;EBV-induced G-protein coupled receptor 1;MIP-3 beta receptor;CD197;CCR7;CMKBR7, EBI1, EVI1; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human CCR7 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone CFO-3 is a rabbit monoclonal antibody that recognizes C-C chemokine receptor type 7 (gene CCR7) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and immunoprecipitation. The predicted molecular weight is 42.9 kDa, and the supplier reports an observed band at about 43 kDa.
CCR7 is a G protein-coupled receptor for the chemokines CCL19 and CCL21. It directs naive T cells, B cells and mature dendritic cells into lymph nodes and other secondary lymphoid organs, so it is a key marker of naive and central memory T cells and a focus of lymphocyte trafficking research.
| Target | C-C chemokine receptor type 7 (gene CCR7; UniProt P32248, human) |
|---|---|
| Clone | CFO-3 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 42.9 kDa |
| Observed MW | 43 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
Samples with a confirmed band (WB): human MCF-7 cells, human K562 cells, rat spleen tissue, rat lung tissue, rat C6 cells, mouse spleen tissue, mouse lung tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Samples with confirmed staining (IHC): human spleen tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Samples with confirmed staining (ICC/IF): HeLa cells.
Immunogen
Synthetic peptide based on the sequence of human CCR7.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places C-C chemokine receptor type 7 in the cell membrane. Tissue expression noted by UniProt (human): Expressed in various lymphoid tissues and activated B- and T-lymphocytes, strongly up-regulated in B-cells infected with Epstein-Barr virus and T-cells infected with herpesvirus 6 or 7. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Lihong Wang et al. (2026) Paeonia lactiflora-derived extracellular vesicles ameliorate diabetic peripheral neuropathy by modulating chemokine-guided immune trafficking in peripheral nerves. Phytomedicine. 10.1016/j.phymed.2026.158346
Wenfeng Li et al. (2022) Pore Size of 3D-Printed Polycaprolactone/Polyethylene Glycol/Hydroxyapatite Scaffolds Affects Bone Regeneration by Modulating Macrophage Polarization and the Foreign Body Response. ACS Applied Materials & Interfaces. 10.1021/acsami.2c02001
Wenfeng Li et al. (2023) Hydrophilic surface-modified 3D printed flexible scaffolds with high ceramic particle concentrations for immunopolarization-regulation and bone regeneration. Biomaterials Science. 10.1039/D3BM00362K
Naoaki Ryo et al. (2026) Adipose tissue inflammation mediated by CCL19 overexpression exacerbates experimental periodontitis via elevated circulating saturated fatty acids and osteopontin in Western-diet-fed mice. Frontiers in Immunology. 10.3389/fimmu.2026.1787572
Haoxuan Wu et al. (2025) CCR7 Promotes Neutrophil Recruitment and N2 Polarization Through Regulation of IL-16 in Head and Neck Squamous Cell Carcinoma. International Dental Journal. 10.1016/j.identj.2025.109311
Hongyi Liu et al. (2020) Autologous bionic tissue for inguinal hernia repair. Journal of Biomedical Materials Research Part a. 10.1002/jbm.a.36907
Zhi-Yong Lei et al. (2018) Efficacy of Aeschynomene indica L. leaves for wound healing and isolation of active constituent. Journal of Ethnopharmacology. 10.1016/j.jep.2018.08.008
YANG Chun-kang et al. (2013) Effect of CO2 pneumoperitoneum on the expression of the chemokine receptors CXCR4 and CCR7 in colorectal carcinoma cellsin vitro. Chinese Medical Journal. 10.3760/cma.j.issn.0366-6999.20123471
