| Field | Specification |
|---|---|
| Target | |
| Alternative names | CCR8; CC chemokine receptor 8; C-C CKR-8; CC-chemokine receptor chemr1; CC-CKR-8; CCR8; CCR-8; CD198; CDw198 antigen; CDw198; chemokine (C-C motif) receptor 8; chemokine (C-C) receptor 8; chemokine (C-C) receptor-like 2; Chemokine receptor-like 1; ChemR1; CKRL1; CKR-L1; CKRL1MGC129966; CMKBR8MGC129973; CMKBRL2C-C chemokine receptor type 8; CY6; GPRCL1; GPRCY6; GPR-CY6; TER-1; TER1CC chemokine receptor CHEMR1 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human CCR8 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ACHG-3 is a rabbit monoclonal antibody that recognizes C-C chemokine receptor type 8 (gene CCR8) in human, mouse and rat samples and is validated for Western blot and ICC/IF. The predicted molecular weight is 40.8 kDa, and the supplier reports an observed band at about 41 kDa.
CCR8 is a G protein-coupled chemokine receptor whose main ligand is CCL1. It is expressed on subsets of regulatory T cells and Th2 cells, and it has drawn strong interest because CCR8 is highly enriched on tumor-infiltrating regulatory T cells.
| Target | C-C chemokine receptor type 8 (gene CCR8; UniProt P51685, human) |
|---|---|
| Clone | ACHG-3 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 40.8 kDa |
| Observed MW | 41 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): human SH-SY5Y cells, human U251 cells, human PC-3 cells, rat PC-12 cells, mouse thymus tissue, mouse RAW264.7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): HeLa cells.
Immunogen
Synthetic peptide based on the sequence of human CCR8.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places C-C chemokine receptor type 8 in the cell membrane. Tissue expression noted by UniProt (human): Expressed in unstimulated CD4(+) and CD8(+) T-cells and polymorphonuclear cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Li Chen et al. (2025) Earlier onset of chemotherapy-induced neuropathic pain in females by ICAM-1–mediated accumulation of perivascular macrophages. Science Advances. 10.1126/sciadv.adu2159
