| Field | Specification |
|---|---|
| Target | |
| Alternative names | C-C chemokine receptor type 9;C-C CKR-9;CC-CKR-9;CCR-9;G-protein coupled receptor 28;GPR-9-6;CDw199;CCR9;GPR28; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human CCR9 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AAO-3 is a rabbit monoclonal antibody that recognizes C-C chemokine receptor type 9 (gene CCR9) in human, mouse and rat samples and is validated for Western blot, ICC/IF and flow cytometry. The predicted molecular weight is 42.0 kDa, and the supplier reports an observed band at about 42 kDa.
CCR9 is a G protein-coupled receptor for the chemokine CCL25 (TECK), which is produced in the thymus and small intestine. It guides developing thymocytes and gut-homing T cells and plasma cells, and it is studied in intestinal immunity and inflammatory bowel disease.
| Target | C-C chemokine receptor type 9 (gene CCR9; UniProt P51686, human) |
|---|---|
| Clone | AAO-3 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 42.0 kDa |
| Observed MW | 42 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:20 |
Samples with a confirmed band (WB): human MOLT-4 cells, human Jurkat cells, human U-87MG cells, human K562 cells, human PANC-1 cells, human A549 cells, human A2780 cells, rat thymus tissue, rat PC-12 cells, mouse thymus tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human CCR9.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places C-C chemokine receptor type 9 in the cell membrane. Tissue expression noted by UniProt (human): Highly expressed in the thymus and low in lymph nodes and spleen. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Min Jiang et al. (2024) Dietary Fiber-Derived Microbial Butyrate Suppresses ILC2-Dependent Airway Inflammation in COPD. Mediators of Inflammation. 10.1155/2024/6263447
