| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Neprilysin;3.4.24.11 ;Atriopeptidase;Common acute lymphocytic leukemia antigen;CALLA;Enkephalinase;Neutral endopeptidase 24.11;NEP;Neutral endopeptidase;Skin fibroblast elastase;SFE;CD10;MME;EPN; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthesized peptide derived from human CD10 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-CD10 MME Rabbit Monoclonal Antibody is an antibody targeting MME. Common applications include WB, Flow Cytometry. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: GEH-13; isotype: Rabbit IgG; reactivity: Human,Mouse,Rat; observed MW: 37 kDa; calculated MW: 85514 MW.
Boster Bio Anti-CD10 MME Rabbit Monoclonal Antibody catalog # M04065. Tested in WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: MME — Neprilysin
- Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: GEH-13; Isotype: Rabbit IgG
- Species reactivity: Human,Mouse,Rat
- Molecular weight guidance: Observed: 37 kDa; Calculated: 85514 MW
Biological background
Protein function (datasheet): Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids (PubMed:15283675, PubMed:8168535). Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond (PubMed:17101991). Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9 (PubMed:15283675). Involved in the degradation of atrial natriuretic factor (ANF) (PubMed:2531377, PubMed:2972276). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers (PubMed:20876573). .
Cellular localization (datasheet): Cell membrane; Single-pass type II membrane protein.
Tissue details (datasheet): Mainly expressed in endothelial cells lining lymphatic vessels. .
Research relevance and current trends
- Commonly studied in contexts related to Angiogenesis,Atherosclerosis,Cancer,Cardiovascular,Cytoskeleton/ECM,ECM Enzymes,Extracellular Matrix,Invasion/Microenvironment,Signal Transduction,Tumor Biomarkers.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Flow cytometry: Quantify target-positive populations in single-cell suspensions; pair with viability and isotype/FMO controls conceptually.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
