| Field | Specification |
|---|---|
| Target | |
| Alternative names | Integrin alpha-E; Integrin alpha M290; CD antigen CD103; Itgae |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived mouse CD103/Itgae recombinant protein (Position: F20-E1061). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes integrin alpha-E (gene Itgae) in mouse and rat samples and is validated for IHC and ELISA. The predicted molecular weight is 128.9 kDa.
Integrin alpha-E (ITGAE, CD103) pairs with integrin beta-7 and binds E-cadherin on epithelial cells. It retains intraepithelial lymphocytes and tissue-resident memory T cells within epithelia, and CD103 is also a marker of a migratory dendritic cell subset in the gut and other tissues.
| Target | Integrin alpha-E (gene Itgae; UniProt Q60677, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 128.9 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 2–5 µg/mL (Mouse, Rat) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with confirmed staining (IHC): mouse colon tissue, rat colon tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant mouse CD103/Itgae fragment (Phe20–Glu1061), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt places integrin alpha-E in the membrane.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Qingmiao Shi et al. (2026) Immunoregulatory mechanisms underlying vitexin-mediated attenuation of concanavalin A-induced liver injury. Phytomedicine. 10.1016/j.phymed.2026.158231
Meng Li et al. (2015) Role of dendritic cell-mediated abnormal immune response in visceral hypersensitivity. International Journal of Clinical and Experimental Medicine. PMID: 26550249
