| Field | Specification |
|---|---|
| Target | |
| Alternative names | Endoglin; Cell surface MJ7/18 antigen; CD105; Eng; Edg; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived mouse CD105 recombinant protein (Position: E27-L226). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes endoglin (gene Eng) in mouse and rat samples and is validated for ELISA, IHC and Western blot. The predicted molecular weight is 70.0 kDa, and the supplier reports an observed band at about 95 kDa.
Endoglin (ENG, CD105) is a transmembrane glycoprotein highly expressed on proliferating vascular endothelial cells. It acts as a coreceptor for TGF-β family ligands, particularly BMP9 signaling through SMAD1, and is needed for normal angiogenesis and vessel integrity. Mutations in ENG cause hereditary hemorrhagic telangiectasia type 1, and CD105 is widely used as a marker of activated endothelium.
| Target | Endoglin (gene Eng; UniProt Q63961, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 70.0 kDa |
| Observed MW | 95 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Mouse) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): rat kidney tissue, mouse kidney tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse kidney tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant mouse CD105 fragment (Glu27–Leu226), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt places endoglin in the cell membrane. Tissue expression noted by UniProt (mouse): Detected on blood vessels (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Lin Shuoqi et al. (2026) Kynurenine promotes angiogenesis through mTOR signaling in head and neck squamous cell carcinoma. Scientific Reports. 10.1038/s41598-026-41141-x
Jun Gao et al. (2023) Effects of norepinephrine‑induced activation of rat vascular adventitial fibroblasts on proliferation and migration of BMSCs involved in vascular remodeling. Experimental and Therapeutic Medicine. 10.3892/etm.2023.11989
Landi Su et al. (2012) Tacrolimus (FK506) prevents early retinal neovascularization in streptozotocin-induced diabetic mice. International Immunopharmacology. 10.1016/j.intimp.2012.09.010
