| Field | Specification |
|---|---|
| Target | |
| Alternative names | Endoglin; CD antigen CD105; ENG; END |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human CD105 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 20E84 is a rabbit monoclonal antibody that recognizes endoglin (gene ENG) in human samples and is validated for Western blot and IHC. The predicted molecular weight is 70.6 kDa, and the supplier reports an observed band at about 90 kDa.
Endoglin (ENG, CD105) is a transmembrane glycoprotein highly expressed on proliferating vascular endothelial cells. It acts as a coreceptor for TGF-β family ligands, particularly BMP9 signaling through SMAD1, and is needed for normal angiogenesis and vessel integrity. Mutations in ENG cause hereditary hemorrhagic telangiectasia type 1, and CD105 is widely used as a marker of activated endothelium.
| Target | Endoglin (gene ENG; UniProt P17813, human) |
|---|---|
| Clone | 20E84 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 70.6 kDa |
| Observed MW | 90 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Immunogen
Synthetic peptide based on the sequence of human CD105.
Reactivity Notes
The supplier lists reactivity with human. UniProt places endoglin in the cell membrane. Tissue expression noted by UniProt (human): Detected on umbilical veil endothelial cells.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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