| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Integrin alpha-M;CD11 antigen-like family member B;CR-3 alpha chain;Cell surface glycoprotein MAC-1 subunit alpha;Leukocyte adhesion receptor MO1;Neutrophil adherence receptor;CD11b;ITGAM;CD11B, CR3A; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human CD11b recombinant protein (Position: F17-T382). Human CD11b shares 79% and 61% amino acid (aa) sequences identity with mouse and rat CD11b, respectively. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of ITGAM (Integrin alpha-M) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-CD11b/ITGAM Antibody Picoband® catalog # PB9140. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E.coli-derived human CD11b recombinant protein (Position: F17-T382). Human CD11b shares 79% and 61% amino acid (aa) sequences identity with mouse and rat CD11b, respectively. (reported region: F17-T382).
- Molecular weight context: reported MW: 45 kDa; calculated MW: 127179 MW
- Reactivity: Human,Mouse,Rat
- Applications: Flow Cytometry, IHC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Integrin alpha-M; Integrin alpha-M. Integrin alpha M (ITGAM) is one protein subunit that forms the heterodimeric integrin alpha-M beta-2 (αMβ2) molecule, also known as macrophage-1 antigen (Mac-1) or complement receptor 3 (CR3). It is mapped to 16p11.2. ITGAM has a role in vascular repair after mechanical arterial injury. It is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. What's more, ITGAM probably recognizes the R-G-D peptide in C3b, and it is also a receptor for fibrinogen, factor X and ICAM1. Functional note: Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Reported localization: Membrane; Single-pass type I membrane protein. Expression/tissue context: Predominantly expressed in monocytes and granulocytes.
Research relevance and current trends
- Cell Adhesion: Researchers commonly examine how ITGAM (Integrin alpha-M) relates to this theme using model systems and orthogonal readouts.
- Cell Type Marker: Researchers commonly examine how ITGAM (Integrin alpha-M) relates to this theme using model systems and orthogonal readouts.
- Cell Type Markers: Researchers commonly examine how ITGAM (Integrin alpha-M) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative ITGAM (Integrin alpha-M) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of ITGAM (Integrin alpha-M) across tissue regions and cell types using matched controls.
- Flow cytometry: quantify target-positive populations and shifts in expression; gating strategy and background staining controls are essential.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Family / similarity context: Belongs to the integrin alpha chain family.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
