| Field | Specification |
|---|---|
| Target | |
| Alternative names | Integrin alpha-M;CD11 antigen-like family member B;CR-3 alpha chain;Cell surface glycoprotein MAC-1 subunit alpha;Leukocyte adhesion receptor MO1;Neutrophil adherence receptor;CD11b;ITGAM;CD11B, CR3A; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human CD11b recombinant protein (Position: F17-T382). Human CD11b shares 79% and 61% amino acid (aa) sequences identity with mouse and rat CD11b, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets integrin alpha-M (gene ITGAM) in human, mouse and rat samples and is validated for flow cytometry, IHC and Western blot. The predicted molecular weight is 127.2 kDa, and the supplier reports an observed band at about 45 kDa.
Integrin alpha-M (ITGAM, CD11b) pairs with integrin beta-2 to form Mac-1, also called complement receptor 3. It binds the complement fragment iC3b, fibrinogen and ICAM-1, supporting adhesion, migration and phagocytosis by monocytes, macrophages and neutrophils. CD11b is one of the most widely used myeloid cell markers.
| Target | Integrin alpha-M (gene ITGAM; UniProt P11215, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 127.2 kDa |
| Observed MW | 45 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Mouse, Rat) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Conditions in the example images (WB): 5–20% gradient SDS-PAGE, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human tonsil tissue, mouse spleen tissue, rat spleen tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with a confirmed signal (flow cytometry): THP-1 cells.
Immunogen
Recombinant human CD11b fragment (Phe17–Thr382), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 79% and 61%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places integrin alpha-M in the cell membrane and membrane raft. Tissue expression noted by UniProt (human): Predominantly expressed in monocytes and granulocytes. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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Guanghui Cui et al. (2009) Rat bone marrow derived mesenchymal progenitor cells support mouse ES cell growth and germ-like cell differentiation. Cell Biology International. 10.1016/j.cellbi.2009.01.008
Lingmin Mu et al. (2014) Expressions of CD11a, CD11b, and CD11c integrin proteins in rats with myocardial hypertrophy. Iranian Journal of Basic Medical Sciences. PMID: 25691929
