| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Heat shock protein beta-8;HspB8;Alpha-crystallin C chain;E2-induced gene 1 protein;Protein kinase H11;Small stress protein-like protein HSP22;HSPB8;CRYAC, E2IG1, HSP22;PP1629; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human CD134 Receptor for TNFSF4/OX40L/GP34. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-TNFRSF4 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone AEHA-20; isotype Rabbit IgG; reactivity: Human. Reported application contexts include WB, Flow (as provided in the source record). Boster Bio Anti-CD134 Monoclonal Antibody catalog # M02495. Tested in WB, Flow Cytometry applications. This antibody reacts with Human.
Key elements and design rationale
- Target: TNFRSF4 (Heat shock protein beta-8).
- Antibody format: Monoclonal; clone AEHA-20; isotype Rabbit IgG.
- Host: Rabbit.
- Species reactivity: Human (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
TNFRSF4 (protein: T-cell surface glycoprotein CD3 zeta chain) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Displays temperature-dependent chaperone activity. Reported cellular localization context: Cytoplasm . Nucleus . Translocates to nuclear foci during heat shock. Tissue expression notes (as provided): Predominantly expressed in skeletal muscle and heart. .
Research relevance and current trends
- Research context keywords from the source record include: Autoimmune,B Cells,Cytokines,Hematopoietic Progenitors,Immune System Diseases,Immunology,Innate Immunity,Lymphoid,Stem Cells,T Cells,T Lymphocytic Lineage,TNF Superfamily.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Flow cytometry: quantify target-positive populations and compare shifts in marker distributions.
Workflow ideas (metafield): Validate TNFRSF4 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect TNFRSF4 expression by Western blot in cell or tissue lysates, Quantify TNFRSF4-positive cells by flow cytometry in single-cell suspensions
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 73 kDa; calculated MW: 21604 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 73 kDa
- Cellular localization (provided): Cytoplasm . Nucleus . Translocates to nuclear foci during heat shock.
- Tissue details (provided): Predominantly expressed in skeletal muscle and heart. .
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
