Anti-CD137/Tnfrsf9 Antibody Picoband®

Overview

This antibody is intended for detection of Tnfrsf9 in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.

Vendor notes: Boster Bio Anti-CD137/Tnfrsf9 Antibody Picoband® catalog # A00867-2. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Key elements and design rationale

• Antibody format: Rabbit Polyclonal Rabbit IgG
• Immunogen / epitope context: E. coli-derived mouse CD137 recombinant protein (Position: V24-L187). (reported region: V24-L187).
• Molecular weight context: reported MW: 65 kDa; calculated MW: nan
• Reactivity: Mouse,Rat
• Applications: ELISA, WB

As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.

Biological background

TNF receptor superfamily member 9. CD137 is a member of the tumor necrosis factor (TNF) receptor family. This receptor contributes to the clonal expansion, survival, and development of T cells. It can also induce proliferation in peripheral monocytes, enhance T cell apoptosis induced by TCR/CD3 triggered activation, and regulate CD28 co-stimulation to promote Th1 cell responses. The expression of this receptor is induced by lymphocyte activation. TRAF adaptor proteins have been shown to bind to this receptor and transduce the signals leading to activation of NF-kappaB. Functional note: Receptor for TNFSF9/4-1BBL. Possibly active during T cell activation. Reported localization: Membrane; Single-pass type I membrane protein. Expression/tissue context: Expressed on the surface of activated T-cells.

Research relevance and current trends

• Apoptosis: Researchers commonly examine how Tnfrsf9 relates to this theme using model systems and orthogonal readouts.
• Associated Proteins: Researchers commonly examine how Tnfrsf9 relates to this theme using model systems and orthogonal readouts.
• Cancer: Researchers commonly examine how Tnfrsf9 relates to this theme using model systems and orthogonal readouts.

Common research applications

• Western blotting: compare relative Tnfrsf9 levels across conditions; band patterns may reflect isoforms and processing.
• ELISA-compatible use: when applicable, interpret signal as relative abundance across sample sets with consistent handling and dilution strategy.

Notes for experimental interpretation

• Specificity notes: No cross reactivity with other proteins.
• Cross-reactivity: No cross-reactivity with other proteins.
• Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
• Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.