| Field | Specification |
|---|---|
| Target | |
| Alternative names | Tumor necrosis factor receptor superfamily member 9; 4-1BB ligand receptor; T-cell antigen 4-1BB; CD137; Tnfrsf9; Cd137; Ila; Ly63 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived mouse CD137 recombinant protein (Position: V24-L187). |
| Molecular weight | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets tumor necrosis factor receptor superfamily member 9 (gene Tnfrsf9) in mouse and rat samples and is validated for ELISA and Western blot. The predicted molecular weight is 27.6 kDa, and the supplier reports an observed band at about 65 kDa.
4-1BB (TNFRSF9, CD137) is a costimulatory receptor induced on activated T cells and NK cells that binds 4-1BB ligand (TNFSF9). Its signaling boosts the survival, cytotoxic activity and metabolic fitness of CD8+ T cells, so it is a major target in antiviral and anti-tumor immunity research and in CAR T-cell design.
| Target | Tumor necrosis factor receptor superfamily member 9 (gene Tnfrsf9; UniProt P20334, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 27.6 kDa |
| Observed MW | 65 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| ELISA (capture antibody) | 1–5 µg/mL |
Samples with a confirmed band (WB): recombinant mouse CD137 protein.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Immunogen
Recombinant mouse CD137 fragment (Val24–Leu187), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt places tumor necrosis factor receptor superfamily member 9 in the cell membrane. Tissue expression noted by UniProt (mouse): Expressed in activated thymocytes, splenic T cells, CD4(+), and CD8(+) T-cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
