| Field | Specification |
|---|---|
| Target | |
| Alternative names | Monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein; CD14; Cd14; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human CD14 recombinant protein (Position: T20-N259). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes monocyte differentiation antigen CD14 in human samples and is validated for IHC, ICC/IF, flow cytometry and ELISA. The predicted molecular weight is 40.1 kDa.
CD14 is a pattern-recognition co-receptor on monocytes and macrophages that, together with LBP, captures bacterial lipopolysaccharide (LPS) and delivers it to the TLR4/MD-2 complex. This triggers NF-κB activation and inflammatory cytokine release, and CD14 is also a standard marker for monocytes in flow cytometry.
| Target | Monocyte differentiation antigen CD14 (gene CD14; UniProt P08571, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 40.1 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with confirmed staining (IHC): human placenta tissue, human tonsil tissue, human lymphadenoma tissue, human renal cells carcinoma tissue, human spleen tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): SiHa cells.
Samples with a confirmed signal (flow cytometry): Caco-2 cells.
Immunogen
Recombinant human CD14 fragment (Thr20–Asn259), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes monocyte differentiation antigen CD14 as secreted and also located in the cell membrane, membrane raft and Golgi apparatus. Tissue expression noted by UniProt (human): Detected on macrophages (at protein level).
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Liu Jun et al. (2018) Pre-vascularization in fibrin Gel/PLGA microsphere scaffolds designed for bone regeneration. NPG Asia Materials. 10.1038/s41427-018-0076-8
Liu Xiuxia et al. (2012) Enhanced expression of the human CD14 protein in tobacco using a 22-kDa alpha-zein signal peptide. Plant Cell Tissue and Organ Culture. 10.1007/s11240-012-0206-x
Dandan Liu et al. (2014) Distinct molecular basis for endothelial differentiation: Gene expression profiles of human mesenchymal stem cells versus umbilical vein endothelial cells. Cellular Immunology. 10.1016/j.cellimm.2014.01.007
Qiang Lu et al. (2012) Expression and clinical significance of extracellular matrix metalloproteinase inducer, EMMPRIN/CD147, in human osteosarcoma. Oncology Letters. 10.3892/ol.2012.981
