| Field | Specification |
|---|---|
| Target | |
| Alternative names | Monocyte differentiation antigen CD14 ;Myeloid cell-specific leucine-rich glycoprotein;CD14;Monocyte differentiation antigen CD14, urinary form;Monocyte differentiation antigen CD14, membrane-bound form;CD14; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human CD14 recombinant protein (Position: N65-D327). Human CD14 shares 69.8% and 66.8% amino acid (aa) sequence identity with mouse and rat CD14, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects monocyte differentiation antigen CD14 in human samples and is validated for IHC and Western blot. The predicted molecular weight is 40.1 kDa, and the supplier reports an observed band at about 50–55 kDa.
CD14 is a pattern-recognition co-receptor on monocytes and macrophages that, together with LBP, captures bacterial lipopolysaccharide (LPS) and delivers it to the TLR4/MD-2 complex. This triggers NF-κB activation and inflammatory cytokine release, and CD14 is also a standard marker for monocytes in flow cytometry.
| Target | Monocyte differentiation antigen CD14 (gene CD14; UniProt P08571, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 40.1 kDa |
| Observed MW | 50–55 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
Samples with a confirmed band (WB): human THP-1 cells, human Raji cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human lung cancer tissue, human liver cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human CD14 fragment (Asn65–Asp327), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 69.8% and 66.8%, respectively.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes monocyte differentiation antigen CD14 as secreted and also located in the cell membrane, membrane raft and Golgi apparatus. Tissue expression noted by UniProt (human): Detected on macrophages (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
