| Field | Specification |
|---|---|
| Target | |
| Alternative names | Low affinity immunoglobulin gamma Fc region receptor III-A; IgG Fc receptor III-A; CD16-II; CD16a antigen; Fc-gamma RIII-alpha; Fc-gamma RIII; Fc-gamma RIIIa; FcRIII; FcRIIIa; FcgammaRIIIA; FcR-10; IgG Fc receptor III-2; CD antigen CD16a; FCGR3A; CD16A; FCG3; FCGR3; IGFR3 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human CD16 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 22F02 is a rabbit monoclonal antibody that recognizes low affinity immunoglobulin gamma Fc region receptor III-A (gene FCGR3A) in human, mouse and rat samples and is validated for Western blot, ICC/IF and flow cytometry. The predicted molecular weight is 29.1 kDa, and the supplier reports an observed band at about 42 kDa.
FcγRIIIA (FCGR3A, CD16a) is a low-affinity receptor for the Fc region of IgG expressed on NK cells, monocytes and macrophages. When it binds antibody-coated target cells, it triggers antibody-dependent cellular cytotoxicity (ADCC). CD16 is a key NK cell marker and is studied in immune defense and therapeutic antibody function.
| Target | Low affinity immunoglobulin gamma Fc region receptor III-A (gene FCGR3A; UniProt P08637, human) |
|---|---|
| Clone | 22F02 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 29.1 kDa |
| Observed MW | 42 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:50 |
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human CD16.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes low affinity immunoglobulin gamma Fc region receptor III-A as secreted and also located in the cell membrane. Tissue expression noted by UniProt (human): Expressed in natural killer cells (at protein level).
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Li Dong et al. (2022) Extra arginine supplementation during the suckling period alleviates weaning stress through the regulation of dendritic cells and Notch2 signaling in piglets. Food & Function. 10.1039/D1FO03720J
Xiangrong Chen et al. (2023) ADAM17 Aggravates the Inflammatory Response by Modulating Microglia Polarization Through the TGF-β1/Smad Pathway Following Experimental Traumatic Brain Injury. Journal of Neurotrauma. 10.1089/neu.2022.0373
Yuanxin Guo et al. (2020) Fingolimod suppressed the chronic unpredictable mild stress-induced depressive-like behaviors via affecting microglial and NLRP3 inflammasome activation. Life Sciences. 10.1016/j.lfs.2020.118582
Liu Fang et al. (2016) IP-10 and fractalkine induce cytotoxic phenotype of murine NK cells. Science Bulletin. 10.1007/s11434-015-0961-2
