| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Alpha-actinin-2;Alpha-actinin skeletal muscle isoform 2;F-actin cross-linking protein;ACTN2; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Gene ID | |
| Host | |
| Immunogen | A synthesized peptide derived from human CD162 A SLe (x) -type proteoglycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. Critical for the initial leukocyte capture. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-SELPLG antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone AEHF-19; isotype Rabbit IgG; reactivity: Human. Reported application contexts include WB, IHC, ICC, IF, IP (as provided in the source record). Boster Bio Anti-CD162 Monoclonal Antibody catalog # M03674-1. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human.
Key elements and design rationale
- Target: SELPLG (Alpha-actinin-2).
- Antibody format: Monoclonal; clone AEHF-19; isotype Rabbit IgG.
- Host: Rabbit.
- Species reactivity: Human (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
SELPLG (protein: T-cell surface glycoprotein CD3 zeta chain) is a commonly studied target in molecular and cellular biology. Functional context (as provided): F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Reported cellular localization context: Cytoplasm, myofibril, sarcomere, Z line . Colocalizes with MYOZ1 and FLNC at the Z-lines of skeletal muscle. Tissue expression notes (as provided): Expressed in both skeletal and cardiac muscle.
Research relevance and current trends
- Research context keywords from the source record include: Immunology,Vascular Inflammation.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
- Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
- Immunoprecipitation (IP): enrich target complexes for downstream immunoblot or interaction analyses.
Workflow ideas (metafield): Validate SELPLG antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect SELPLG expression by Western blot in cell or tissue lysates, Detect SELPLG in FFPE tissue sections by immunohistochemistry, Localize SELPLG by immunofluorescence/immunocytochemistry in cultured cells, Enrich SELPLG by immunoprecipitation from lysates for downstream analysis
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 103 kDa; calculated MW: 103854 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 103 kDa
- Cellular localization (provided): Cytoplasm, myofibril, sarcomere, Z line . Colocalizes with MYOZ1 and FLNC at the Z-lines of skeletal muscle.
- Tissue details (provided): Expressed in both skeletal and cardiac muscle.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
