| Field | Specification |
|---|---|
| Target | |
| Alternative names | Scavenger receptor cysteine-rich type 1 protein M130;Hemoglobin scavenger receptor;CD163;Soluble CD163;sCD163;CD163;M130; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human CD163 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ABCC-3 is a rabbit monoclonal antibody that recognizes scavenger receptor cysteine-rich type 1 protein M130 (gene CD163) in human samples and is validated for Western blot, IHC and immunoprecipitation. The predicted molecular weight is 125.5 kDa, and the supplier reports an observed band at about 150 kDa.
CD163 is a scavenger receptor on monocytes and macrophages that takes up hemoglobin–haptoglobin complexes, protecting tissues from free hemoglobin and supporting iron recycling. It is widely used as a marker of anti-inflammatory (M2-like) macrophages, and its soluble form is measured as a readout of macrophage activation.
| Target | Scavenger receptor cysteine-rich type 1 protein M130 (gene CD163; UniProt Q86VB7, human) |
|---|---|
| Clone | ABCC-3 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 125.5 kDa |
| Observed MW | 150 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunoprecipitation | 1:30 |
Samples with a confirmed band (WB): human fetal kidney.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): mouse liver tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human CD163.
Reactivity Notes
The supplier lists reactivity with human. UniProt places scavenger receptor cysteine-rich type 1 protein M130 in the [Soluble CD163]: Secreted and SUBCELLULAR LOCATION: Cell membrane. Tissue expression noted by UniProt (human): Expressed in monocytes and mature macrophages such as Kupffer cells in the liver, red pulp macrophages in the spleen, cortical macrophages in the thymus, resident bone marrow macrophages and meningeal macrophages of the central nervous system. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Ester Handayani Lodra et al. (2023) Fenugreek Seed Ethanolic Extract Improves Alveolar Bone Parameters by Attenuating Inflammation in Ovariectomized Rats. Journal of Inflammation Research. 10.2147/JIR.S428791
Huaqiang Yang et al. (2018) CD163 knockout pigs are fully resistant to highly pathogenic porcine reproductive and respiratory syndrome virus. Antiviral Research. 10.1016/j.antiviral.2018.01.004
