| Field | Specification |
|---|---|
| Target | |
| Alternative names | CD166 antigen; Activated leukocyte cell adhesion molecule; CD antigen CD166; ALCAM; MEMD |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human CD166 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ADGA-1 is a rabbit monoclonal antibody that recognizes CD166 antigen (gene ALCAM) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 65.1 kDa, and the supplier reports an observed band at about 100 kDa.
ALCAM (CD166) is an immunoglobulin superfamily adhesion molecule that mediates cell-cell contacts both by binding itself and by binding CD6 on T cells. The ALCAM-CD6 interaction supports immunological synapse formation and T cell activation, and ALCAM is also studied in neural development, stem cells and cancer.
| Target | CD166 antigen (gene ALCAM; UniProt Q13740, human) |
|---|---|
| Clone | ADGA-1 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 65.1 kDa |
| Observed MW | 100 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:40 |
| Flow cytometry | 1:60 |
Samples with a confirmed band (WB): Daudi cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human CD166.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places CD166 antigen in the cell membrane and cell projection. Tissue expression noted by UniProt (human): Detected on hematopoietic stem cells derived from umbilical cord blood. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Shengsheng Li et al. (2026) LncRNA WFDC21P mediates the tobacco carcinogen induced malignant transformation of human normal lung epithelial cells by regulating tumor stemness. Food and Chemical Toxicology. 10.1016/j.fct.2026.116116
