| Field | Specification |
|---|---|
| Target | |
| Alternative names | B-lymphocyte antigen CD19;B-lymphocyte surface antigen B4;Differentiation antigen CD19;T-cell surface antigen Leu-12;CD19;CD19; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human CD19 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AOEB-3 is a rabbit monoclonal antibody that recognizes B-lymphocyte antigen CD19 in human, mouse and rat samples and is validated for Western blot and flow cytometry. The predicted molecular weight is 61.1 kDa, and the supplier reports an observed band at about 95 kDa.
CD19 is a B-cell co-receptor that forms a complex with CD21 and CD81 and lowers the threshold for B-cell receptor signaling. It is expressed from early B-cell development until plasma cell differentiation, making it the standard pan-B-cell marker and a well-known target of CAR T-cell therapy research.
| Target | B-lymphocyte antigen CD19 (gene CD19; UniProt P15391, human) |
|---|---|
| Clone | AOEB-3 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 61.1 kDa |
| Observed MW | 95 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:1000 |
|---|---|
| Flow cytometry | 1:50 |
Immunogen
Synthetic peptide based on the sequence of human CD19.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places B-lymphocyte antigen CD19 in the cell membrane and membrane raft. Tissue expression noted by UniProt (human): Detected on marginal zone and germinal center B cells in lymph nodes.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
