| Field | Specification |
|---|---|
| Target | |
| Alternative names | T-cell surface glycoprotein CD1b; CD1b; CD1B |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human CD1B recombinant protein (Position: D78-Y110). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets T-cell surface glycoprotein CD1b in human, mouse and rat samples and is validated for Western blot and ELISA. The predicted molecular weight is 36.9 kDa, and the supplier reports an observed band at about 40 kDa.
CD1b is a member of the CD1 family of antigen-presenting molecules that display lipid and glycolipid antigens to T cells. Its large binding groove accommodates long-chain mycobacterial lipids such as mycolic acids, so it is studied in T-cell responses to tuberculosis and lipid antigen presentation.
| Target | T-cell surface glycoprotein CD1b (gene CD1B; UniProt P29016, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 36.9 kDa |
| Observed MW | 40 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human hcct tissue, human MOLT-4 tissue, human Jurkat cells, rat thymus tissue, mouse thymus tissue, mouse RAW264.7 cells, mouse Ana-1 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human CD1B fragment (Asp78–Tyr110), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places T-cell surface glycoprotein CD1b in the cell membrane, endosome membrane and lysosome membrane. Tissue expression noted by UniProt (human): Expressed on cortical thymocytes, on certain T-cell leukemias, and in various other tissues. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yabasin Iddrisu B. et al. (2018) Cisatracurium Retards Cell Migration and Invasion Upon Upregulation of p53 and Inhibits the Aggressiveness of Colorectal Cancer. Frontiers in Physiology. 10.3389/fphys.2018.00941
