| Field | Specification |
|---|---|
| Target | |
| Alternative names | T-cell surface glycoprotein CD1c; CD1c; CD1C |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human CD1c recombinant protein (Position: K73-L287). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes T-cell surface glycoprotein CD1c in human samples and is validated for Western blot. The predicted molecular weight is 37.7 kDa, and the supplier reports an observed band at about 62 kDa.
CD1c is a CD1 family protein that presents lipid and glycolipid antigens, including mycobacterial lipopeptides, to T cells. It is expressed on B cells and a major subset of dendritic cells, where it serves as a marker of type 2 conventional dendritic cells (cDC2).
| Target | T-cell surface glycoprotein CD1c (gene CD1C; UniProt P29017, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 37.7 kDa |
| Observed MW | 62 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|
Samples with a confirmed band (WB): human placenta tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Immunogen
Recombinant human CD1c fragment (Lys73–Leu287), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places T-cell surface glycoprotein CD1c in the cell membrane, endosome membrane and lysosome. Tissue expression noted by UniProt (human): Expressed on cortical thymocytes, on certain T-cell leukemias, and in various other tissues. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
