| Field | Specification |
|---|---|
| Target | |
| Alternative names | T-cell surface antigen CD2;LFA-2;LFA-3 receptor;Lymphocyte antigen 37;Ly-37;T-cell surface antigen T11/Leu-5;CD2;Cd2;Ly-37; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived mouse CD2 recombinant protein (Position: R23-S203). Mouse CD2 shares 49.7% and 73.3% amino acid (aa) sequence identity with human and rat CD2, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets T-cell surface antigen CD2 in mouse samples and is validated for IHC and Western blot. The predicted molecular weight is 38.4 kDa, and the supplier reports an observed band at about 58 kDa.
CD2 is a T-cell and NK-cell surface adhesion molecule that binds CD58 (LFA-3) in humans and CD48 in rodents. This interaction strengthens contact between T cells and antigen-presenting or target cells, and CD2 also contributes co-stimulatory signals for T-cell activation.
| Target | T-cell surface antigen CD2 (gene Cd2; UniProt P08920, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 38.4 kDa |
| Observed MW | 58 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL (Mouse) |
|---|---|
| Western blot | 0.1–0.5 µg/mL (Mouse) |
Samples with a confirmed band (WB): mouse cardiac muscle tissue, HEPA cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse lymphaden tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant mouse CD2 fragment (Arg23–Ser203), expressed in E. coli. Sequence identity with the human and rat orthologs: 49.7% and 73.3%, respectively.
Reactivity Notes
The supplier lists reactivity with mouse. UniProt places T-cell surface antigen CD2 in the cell membrane. Tissue expression noted by UniProt (mouse): Detected in thymus and spleen. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
