| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | T-cell surface antigen CD2;LFA-2;LFA-3 receptor;Lymphocyte antigen 37;Ly-37;T-cell surface antigen T11/Leu-5;CD2;Cd2;Ly-37; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E. coli-derived mouse CD2 recombinant protein (Position: R23-S203). Mouse CD2 shares 49.7% and 73.3% amino acid (aa) sequence identity with human and rat CD2, respectively. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of Cd2 (T-cell surface antigen CD2) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-CD2/CD2 Antibody Picoband® catalog # PB9929. Tested in IHC, WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E. coli-derived mouse CD2 recombinant protein (Position: R23-S203). Mouse CD2 shares 49.7% and 73.3% amino acid (aa) sequence identity with human and rat CD2, respectively. (reported region: R23-S203).
- Molecular weight context: reported MW: 58 kDa; calculated MW: 38415 MW
- Reactivity: Mouse
- Applications: IHC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
T-cell surface antigen CD2; T-cell surface antigen CD2. CD2 (cluster of differentiation 2) is a cell adhesion molecule found on the surface of T cells and natural killer (NK) cells. It has also been called T-cell surface antigen T11/Leu-5, LFA-2, LFA-3 receptor, erythrocyte receptor and rosette receptor. Monoclonal antibodies ed against CD2 inhibit the formation of rosettes with sheep erythrocytes, indicating that CD2 is the erythrocyte receptor or is closely associated with it. CD2 is one of the earliest T-cell markers, being present on more than 95% of thymocytes. Due to its structural characteristics, CD2 is a member of the immunoglobulin superfamily; it possesses two immunoglobulin-like domains in its extracellular portion. The localization of CD2 to 1p13 is established by in situ hybridization. CD2 interacts with other adhesion molecules, such as lymphocyte function-associated antigen-3 (LFA-3/CD58) in humans, or CD48 in rodents, which are expressed on the surfaces of other cells. With the use of transgenic mice, such an LCR is identified within the 3-prime flanking region of the human CD2 gene. Functional note: CD2 interacts with lymphocyte function-associated antigen (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T- cells, the cytoplasmic domain is implicated in the signaling function. Reported localization: Membrane; Single-pass type I membrane protein. Expression/tissue context: Expressed in the temporal lobe, frontal lobe, parietal lobe, hippocampus, and cerebellum. Also found in kidney, lung, liver, heart, skeletal muscle, placenta. .
Research relevance and current trends
- Stem Cells: Researchers commonly examine how Cd2 (T-cell surface antigen CD2) relates to this theme using model systems and orthogonal readouts.
- T Cells: Researchers commonly examine how Cd2 (T-cell surface antigen CD2) relates to this theme using model systems and orthogonal readouts.
- T Lymphocytic Lineage: Researchers commonly examine how Cd2 (T-cell surface antigen CD2) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative Cd2 (T-cell surface antigen CD2) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of Cd2 (T-cell surface antigen CD2) across tissue regions and cell types using matched controls.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
