| Field | Specification |
|---|---|
| Target | |
| Alternative names | Complement receptor type 2;Cr2;Complement C3d receptor;Epstein-Barr virus receptor;EBV receptor;CD21;CR2;C3DR; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human CD21 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone FHB-3 is a rabbit monoclonal antibody that recognizes complement receptor type 2 (gene CR2) in human and mouse samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 112.9 kDa, and the supplier reports an observed band at about 155 kDa.
Complement receptor 2 (CR2, CD21) is expressed on B cells and follicular dendritic cells and binds C3d on complement-tagged antigens. As part of the CD19–CD21–CD81 co-receptor complex it lowers the threshold for B-cell activation, and it is also the receptor used by Epstein-Barr virus to enter B cells.
| Target | Complement receptor type 2 (gene CR2; UniProt P20023, human) |
|---|---|
| Clone | FHB-3 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 112.9 kDa |
| Observed MW | 155 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:1000–1:5000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
| Flow cytometry | 1:50 |
Samples with confirmed staining (IHC): human spleen tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human CD21.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt places complement receptor type 2 in the cell membrane. Tissue expression noted by UniProt (human): Mature B-lymphocytes, T-lymphocytes, pharyngeal epithelial cells, astrocytes and follicular dendritic cells of the spleen. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
