Anti-CD22/Siglec 2 Rabbit Monoclonal Antibody

Overview

Anti-CD22/Siglec 2 Rabbit Monoclonal Antibody is an antibody targeting CD22. Common applications include WB, IHC. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: CBO-3; isotype: Rabbit IgG; reactivity: Human,Mouse,Rat; observed MW: 140 kDa; calculated MW: 95348 MW.

Boster Bio Anti-CD22/Siglec 2 Rabbit Monoclonal Antibody catalog # M01572-1. Tested in WB, IHC application. This antibody reacts with Human, Mouse, Rat.

Key elements and design rationale

• Target: CD22 — B-cell receptor CD22
• Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: CBO-3; Isotype: Rabbit IgG
• Species reactivity: Human,Mouse,Rat
• Molecular weight guidance: Observed: 140 kDa; Calculated: 95348 MW

Biological background

Protein function (datasheet): Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules.

Cellular localization (datasheet): Cell membrane; Single-pass type I membrane protein.

Tissue details (datasheet): B-lymphocytes.

Research relevance and current trends

• Commonly studied in contexts related to Adaptive Immunity,B Cells,Cancer,Hematopoietic Progenitors,Immunology,Lymphoid,Stem Cells,Tumor Antigens,Tumor Biomarkers,Tumor Immunology,Tumor-Associated Antigens.
• Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
• Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).

Common research applications

• Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
• Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.

Notes for experimental interpretation

• Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
• Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.

As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.