Anti-CD25 IL2RA Monoclonal Antibody

Overview

This product is an anti-IL2RA antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone AFGC-9; isotype Rabbit IgG; reactivity: Human. Reported application contexts include IHC, ICC, IF (as provided in the source record). Boster Bio Anti-CD25 IL2RA Monoclonal Antibody catalog # M00214-1. Tested in IHC, ICC/IF applications. This antibody reacts with Human.

Key elements and design rationale

• Target: IL2RA (Glial fibrillary acidic protein).
• Antibody format: Monoclonal; clone AFGC-9; isotype Rabbit IgG.
• Host: Rabbit.
• Species reactivity: Human (confirm in your model system with appropriate controls).

This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.

Biological background

IL2RA (protein: P2X purinoceptor 1) is a commonly studied target in molecular and cellular biology. Functional context (as provided): GFAP, a class-III intermediate filament, is a cell- specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. Reported cellular localization context: Cytoplasm . Associated with intermediate filaments. Tissue expression notes (as provided): Expressed in cells lacking fibronectin. .

Research relevance and current trends

• Research context keywords from the source record include: Adaptive Immunity,Cytokines,Immunology,Innate Immunity,Interleukins,T Cells.
• Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
• Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.

Common research applications

• Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
• Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.

Workflow ideas (metafield): Validate IL2RA antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect IL2RA in FFPE tissue sections by immunohistochemistry, Localize IL2RA by immunofluorescence/immunocytochemistry in cultured cells

Notes for experimental interpretation

• Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
• Apparent molecular weight may vary by sample type and processing (observed MW: 30 kDa; calculated MW: 49880 MW).
• Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.

Additional product details (from the source record)

• Molecular weight (observed): 30 kDa
• Cellular localization (provided): Cytoplasm . Associated with intermediate filaments.
• Tissue details (provided): Expressed in cells lacking fibronectin. .

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.