| Field | Specification |
|---|---|
| Target | |
| Alternative names | CD27 antigen; CD27L receptor; T-cell activation antigen CD27; T14; Tumor necrosis factor receptor superfamily member 7; CD antigen CD27; CD27; TNFRSF7 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human CD27 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 20C54 is a rabbit monoclonal antibody that recognizes CD27 antigen in human and rat samples and is validated for Western blot, ICC/IF and flow cytometry. The predicted molecular weight is 29.1 kDa, and the supplier reports an observed band at about 55 kDa.
CD27 is a TNF receptor superfamily co-stimulatory receptor on T cells, NK cells and B cells that binds CD70. CD70–CD27 signaling supports T-cell activation, survival and memory formation, and CD27 is a commonly used marker for memory B cells and T-cell differentiation states.
| Target | CD27 antigen (gene CD27; UniProt P26842, human) |
|---|---|
| Clone | 20C54 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 29.1 kDa |
| Observed MW | 55 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:50 |
Immunogen
Synthetic peptide based on the sequence of human CD27.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places CD27 antigen in the cell membrane. Tissue expression noted by UniProt (human): Found in most T-lymphocytes.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
