| Field | Specification |
|---|---|
| Target | |
| Alternative names | CD2-associated protein; Adapter protein CMS; Cas ligand with multiple SH3 domains; CD2AP |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human CD2AP recombinant protein (Position: K253-K337). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects CD2-associated protein (gene CD2AP) in human, mouse and rat samples and is validated for ELISA, flow cytometry, immunoprecipitation, ICC/IF, IHC and Western blot. The predicted molecular weight is 71.5 kDa, and the supplier reports an observed band at about 80 kDa.
CD2-associated protein (CD2AP) is an adaptor that links membrane proteins to the actin cytoskeleton and regulates endocytic trafficking. It is essential for the kidney podocyte slit diaphragm, where it associates with nephrin, and it is also studied as a genetic risk locus in Alzheimer′s disease.
| Target | CD2-associated protein (gene CD2AP; UniProt Q9Y5K6, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 71.5 kDa |
| Observed MW | 80 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL |
| Immunoprecipitation | 0.5–2 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human Jurkat cells, human A431 cells, human HeLa cells, human K562 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse kidney tissue, rat kidney tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): HeLa cells.
Samples with a confirmed result (IP): Jurkat cells.
Samples with a confirmed signal (flow cytometry): K56 cells.
Immunogen
Recombinant human CD2AP fragment (Lys253–Lys337), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places CD2-associated protein in the cytoplasm, cell projection and cell junction. Tissue expression noted by UniProt (human): Widely expressed in fetal and adult tissues. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Shuhui Zhou et al. (2024) Shensu IV maintains the integrity of the glomerular filtration barrier and exerts renal protective effects by regulating endogenous hydrogen sulfide levels. Frontiers in Pharmacology. 10.3389/fphar.2024.1447249
