Anti-CD3 Epsilon antibody [145-2C11] {AF488} (STJA0000580)

What it means: Monoclonal antibodies originate from a single hybridoma clone that recognizes one defined epitope on the target antigen — making Monoclonal antibodies uniquely consistent between lots.

Why it matters:

• Monoclonal antibodies offer lot-to-lot reproducibility that is critical for longitudinal studies, quantitative assays, and any experimental design requiring matched performance across timepoints or collaborating labs.
• The defined single-epitope binding profile enables reliable pairing with a non-competing capture antibody for sandwich ELISA, proximity ligation, and flow cytometry multiplex panels.

How to interpret here: A single, well-optimized titration for this Monoclonal antibody transfers reliably across experiments. Confirm whether the target epitope is preserved under your fixation or denaturation conditions before proceeding with IHC or WB applications.

What it means: This antibody was raised in Armenian Hamster, producing Armenian Hamster-derived IgG antibodies with species-specific secondary antibody compatibility.

Why it matters:

• The Armenian Hamster host species determines which secondary antibody you need for indirect detection — select an anti-Armenian Hamster IgG secondary matched to your detection label (HRP, fluorochrome, or biotin).
• Use secondaries specifically validated for Armenian Hamster IgG. Verify cross-adsorption status if your sample matrix contains endogenous Armenian Hamster immunoglobulins.

How to interpret here: Confirm availability of an anti-Armenian Hamster secondary antibody in your lab before ordering. For multiplex IF, pair this Armenian Hamster-raised antibody with antibodies from a different host species to avoid secondary antibody cross-reactivity.

What it means: This antibody has validated reactivity to Mouse antigen, confirming it binds the Mouse ortholog of the target protein in immunoassay conditions.

Why it matters:

• Reactivity to Mouse is confirmed through experimental validation — not merely predicted from sequence homology — ensuring reliable performance in Mouse-derived samples such as cell lysates, tissues, or serum.
• Use of this antibody with non-Mouse samples requires independent validation; predicted cross-reactivity should always be confirmed empirically.

How to interpret here: Ensure your experimental samples are of Mouse origin. If you require reactivity to additional species, contact our team to identify a cross-reactive alternative.

What it means: AF488 format indicates this antibody carries a specific label enabling direct detection in compatible assay systems.

Why it matters:

• The AF488 conjugate eliminates the secondary antibody incubation step, shortening your protocol and reducing non-specific background from secondary reagents.
• Directly labeled antibodies like this AF488 format are particularly advantageous in multiplex panels where multiple primary antibodies from the same host species must be distinguished simultaneously.

How to interpret here: Confirm instrument or substrate compatibility with the AF488 label. Store per datasheet to preserve label integrity — light-sensitive conjugates require protection from light.

What it means: IgG is the immunoglobulin class and subclass of this antibody, defining its constant-region structure and effector functions.

Why it matters:

• Isotype IgG determines which secondary antibody constant-region specificities are compatible — isotype-specific anti-IgG1 or anti-IgG2a secondaries enable discrimination of co-stained antibodies from the same host species in multiplex experiments.
• For functional assays, isotype identity guides selection of Fc-receptor binding controls and isotype-matched negative controls required for rigorous experimental design.

How to interpret here: Use a negative control of the same isotype (IgG) to assess non-specific binding in your assay. If performing multiplex staining with multiple antibodies from the same species, select isotype-specific secondary antibodies to resolve each IgG signal independently.

What it means: 145-2C11 is the unique clone designation of this monoclonal antibody, identifying the specific hybridoma lineage that defines its epitope and performance characteristics.

Why it matters:

• Clone 145-2C11 allows precise cross-referencing with published literature — if peer-reviewed studies cite this specific clone, its performance in your assay context is already independently validated.
• Recording clone 145-2C11 in your methods section enables collaborators and reviewers to replicate your exact reagent, satisfying increasingly stringent journal antibody reporting requirements (RRID registration, clone number disclosure).

How to interpret here: Search clone 145-2C11 in published literature and antibody validation databases to review independent performance data. Always record the full clone designation in experimental notes for complete reproducibility documentation.

What it means: This antibody has been experimentally validated for 1 application: Flow Cytometry.

Why it matters:

• Flow Cytometry
• Each listed application represents an independently optimized protocol — not a theoretical prediction — giving you confidence that the antibody will perform reliably when used at the recommended conditions for that technique.

How to interpret here: Select the application that matches your assay (Flow Cytometry) and refer to the datasheet for the validated dilution range specific to each technique. Using this antibody outside the listed validated applications requires independent optimization and performance verification.