| Field | Specification |
|---|---|
| Target | |
| Alternative names | T-cell surface glycoprotein CD3 epsilon chain;T-cell surface antigen T3/Leu-4 epsilon chain;CD3e;CD3E;T3E; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human CD3 epsilon recombinant protein (Position: D23-I207). Human CD3 epsilon shares 65% amino acid (aa) sequence identity with mouse CD3 epsilon. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets T-cell surface glycoprotein CD3 epsilon chain (gene CD3E) in chicken, human, mouse and rat samples and is validated for ICC/IF, IHC and Western blot. The predicted molecular weight is 23.1 kDa, and the supplier reports an observed band at about 23 kDa.
CD3ε (CD3E) is a core invariant chain of the T-cell receptor–CD3 complex, pairing with CD3γ or CD3δ. It transmits antigen-recognition signals through its ITAM motif, and anti-CD3ε antibodies are the standard tools for identifying and activating T cells.
| Target | T-cell surface glycoprotein CD3 epsilon chain (gene CD3E; UniProt P07766, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Chicken, Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 23.1 kDa |
| Observed MW | 23 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Mouse, Rat, Chicken) |
| IHC (frozen sections) | 0.5–1 µg/mL (Mouse, Rat) |
| Immunocytochemistry / Immunofluorescence | 0.5–1 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | 2 µg/mL (Human, Mouse, Rat) |
Samples with a confirmed band (WB): human MOLT-4 cells, human Jurkat cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human tonsil tissue, mouse spleen tissue, rat spleen tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Samples with confirmed staining (IHC): rat spleen tissue, mouse spleen tissue.
Samples with confirmed staining (ICC/IF): human tonsil tissue.
Immunogen
Recombinant human CD3 epsilon fragment (Asp23–Ile207), expressed in E. coli. Sequence identity with the mouse ortholog: 65%.
Reactivity Notes
The supplier lists reactivity with chicken, human, mouse and rat. UniProt places T-cell surface glycoprotein CD3 epsilon chain in the cell membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Zhong Wenbin et al. (2022) An acquired phosphatidylinositol 4-phosphate transport initiates T-cell deterioration and leukemogenesis. Nature Communications. 10.1038/s41467-022-32104-7
Zhao Qin et al. (2020) Dual-Wavelength Photosensitive Nano-in-Micro Scaffold Regulates Innate and Adaptive Immune Responses for Osteogenesis. Nano-Micro Letters. 10.1007/s40820-020-00540-z
Yu Jianpeng et al. (2025) Resilience and Vulnerabilities of Tumor Cells under Purine Shortage Stress. Clinical Cancer Research. 10.1158/1078-0432.CCR-25-1667
Kun Tu et al. (2021) Combination of Chidamide-Mediated Epigenetic Modulation with Immunotherapy: Boosting Tumor Immunogenicity and Response to PD-1/PD-L1 Blockade. ACS Applied Materials & Interfaces. 10.1021/acsami.1c08290
Yidi Qu et al. (2023) The involvement of Th1 cell differentiation in the anti-tumor effect of purified polysaccharide from Sanghuangporus vaninii in colorectal cancer via multi-omics analysis. International Journal of Biological Macromolecules. 10.1016/j.ijbiomac.2023.123927
Jianjun Ma et al. (2022) CXCR4 knockout induces neuropathological changes in the MPTP-lesioned model of Parkinson's disease. Biochimica et Biophysica ACTA-Molecular Basis of Disease. 10.1016/j.bbadis.2022.166597
Zhibin Niu et al. (2026) The construction of recombinant DNA vaccine pVAX-ROP27-IL-2-IFN-γ and its immune enhancement effect against Eimeria tenella. Poultry Science. 10.1016/j.psj.2026.106466
Guanqi Liu et al. (2026) Immunological Features of Peri-Implant Soft Tissue After Healing Abutment Dislodgement: A Comparative Human Study. International Dental Journal. 10.1016/j.identj.2026.109521
Chan Dai et al. (2023) IL-33 Contributes to the Pathological Changes of Hair Follicles in Psoriasis: A Potential Target for Psoriatic Alopecia. Clinical Cosmetic and Investigational Dermatology. 10.2147/CCID.S403075
Bin Chen et al. (2015) Effectiveness of immune therapy combined with chemotherapy on the immune function and recurrence rate of cervical cancer. Experimental and Therapeutic Medicine. 10.3892/etm.2015.2217
