| Field | Specification |
|---|---|
| Target | |
| Alternative names | Tumor necrosis factor receptor superfamily member 8; CD30L receptor; Ki-1 antigen; Lymphocyte activation antigen CD30; CD antigen CD30; TNFRSF8; CD30; D1S166E |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human CD30 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 18T10 is a rabbit monoclonal antibody that recognizes tumor necrosis factor receptor superfamily member 8 (gene TNFRSF8) in human samples and is validated for Western blot and IHC. The predicted molecular weight is 63.7 kDa, and the supplier reports an observed band at about 120 kDa.
CD30 (TNFRSF8) is a TNF receptor family member found on activated T and B lymphocytes. Binding of its ligand CD30L (TNFSF8) activates NF-κB and influences lymphocyte growth and survival. CD30 is a well-known marker of Hodgkin lymphoma Reed-Sternberg cells and anaplastic large cell lymphoma, and it is widely used in lymphoma research.
| Target | Tumor necrosis factor receptor superfamily member 8 (gene TNFRSF8; UniProt P28908, human) |
|---|---|
| Clone | 18T10 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 63.7 kDa |
| Observed MW | 120 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): A431 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human CD30.
Reactivity Notes
The supplier lists reactivity with human. Tissue expression noted by UniProt (human): Detected in alveolar macrophages (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
