| Field | Specification |
|---|---|
| Target | |
| Alternative names | Platelet endothelial cell adhesion molecule;PECAM-1;EndoCAM;GPIIA';PECA1;CD31;PECAM1; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human CD31 recombinant protein (Position: Q28-G382). Human CD31 shares 65% and 68% amino acid (aa) sequences identity with mouse and rat CD31, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets platelet endothelial cell adhesion molecule (gene PECAM1) in human samples and is validated for flow cytometry, IHC and Western blot. The predicted molecular weight is 82.5 kDa; the supplier reports observed bands at 90 kDa, 130 kDa.
PECAM-1 (CD31) is an immunoglobulin-superfamily adhesion receptor concentrated at endothelial cell junctions and also found on platelets and leukocytes. Through homophilic binding it supports endothelial junction integrity and the passage of leukocytes across the vessel wall during inflammation, and it is the most widely used endothelial marker for staining blood vessels.
| Target | Platelet endothelial cell adhesion molecule (gene PECAM1; UniProt P16284, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 82.5 kDa |
| Observed MW | 90 kDa, 130 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells |
Samples with a confirmed band (WB): human Jurkat cells, human Raji cells, human THP-1 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human hepatocellular carcinoma tissue, human placenta tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with a confirmed signal (flow cytometry): Jurkat cells.
Immunogen
Recombinant human CD31 fragment (Gln28–Gly382), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 65% and 68%, respectively.
Reactivity Notes
The supplier lists reactivity with human. UniProt places platelet endothelial cell adhesion molecule in the cell membrane. Tissue expression noted by UniProt (human): Expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Shen Song et al. (2021) Antibacterial polyvinyl alcohol/bacterial cellulose/nano-silver hydrogels that effectively promote wound healing. Materials Science & Engineering C-Materials for Biological Applications. 10.1016/j.msec.2021.112171
Huang Qianqian et al. (2022) Asprosin Exacerbates Endothelium Inflammation Induced by Hyperlipidemia Through Activating IKKβ-NF-κBp65 Pathway. Inflammation. 10.1007/s10753-022-01761-7
Jun Chen et al. (2011) In Vivo Serial MR Imaging of Magnetically Labeled Endothelial Progenitor Cells Homing to the Endothelium Injured Artery in Mice. PLoS One. 10.1371/journal.pone.0020790
Huiyu Xu et al. (2019) Exosomes derived from adipose tissue, bone marrow, and umbilical cord blood for cardioprotection after myocardial infarction. Journal of Cellular Biochemistry. 10.1002/jcb.27399
