| Field | Specification |
|---|---|
| Target | |
| Alternative names | Platelet endothelial cell adhesion molecule; PECAM-1; EndoCAM; GPIIA'; PECA1; CD antigen CD31; PECAM1 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human CD31 recombinant protein (Position: Q28-G382). Human CD31 shares 65% and 68% amino acid (aa) sequences identity with mouse and rat CD31, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 2D4 is a mouse monoclonal antibody that recognizes platelet endothelial cell adhesion molecule (gene PECAM1) in human samples and is validated for Western blot and IHC. The predicted molecular weight is 82.5 kDa, and the supplier reports an observed band at about 130 kDa.
PECAM-1 (CD31) is an immunoglobulin-superfamily adhesion receptor concentrated at endothelial cell junctions and also found on platelets and leukocytes. Through homophilic binding it supports endothelial junction integrity and the passage of leukocytes across the vessel wall during inflammation, and it is the most widely used endothelial marker for staining blood vessels.
| Target | Platelet endothelial cell adhesion molecule (gene PECAM1; UniProt P16284, human) |
|---|---|
| Clone | 2D4 |
| Host / Clonality / Isotype | Mouse / Monoclonal / Mouse IgG2b |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 82.5 kDa |
| Observed MW | 130 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human) |
| IHC (frozen sections) | 0.5–1 µg/mL (Human) |
Samples with a confirmed band (WB): human Jurkat cells, human placenta tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): human lung cancer tissue, human mammary cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (IHC): human placenta tissue.
Immunogen
Recombinant human CD31 fragment (Gln28–Gly382), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 65% and 68%, respectively.
Reactivity Notes
The supplier lists reactivity with human. UniProt places platelet endothelial cell adhesion molecule in the cell membrane. Tissue expression noted by UniProt (human): Expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yan Zhang et al. (2009) Combined endostatin and TRAIL gene transfer suppresses human hepatocellular carcinoma growth and angiogenesis in nude mice. Cancer Biology & Therapy. 10.4161/cbt.8.5.7687
