Anti-CD33 Antibody Picoband®

SKU:BHA21000871
Research Validated
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Suppliers
Boster Bio
Boster Bio
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Overview
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Anti-Cd33 antibody (Rabbit polyclonal, Rabbit IgG). Validated for immunohistochemistry and Western blot. Reactivity: Mouse. Recognizes myeloid cell surface antigen CD33. Observed band in Western blot: about 26 kDa.
Target Cd33
Host Rabbit
Reactivity Mouse
Isotype Rabbit IgG
Application(s) IHC, WB
Options selector
Catalog no. Size Conjugation
RP1105 100 ug/vial
Available Options

Select the variant that best fits your experiment.

  • Options:
    • 100 ug/vial / Carrier Free, Unconjugated
      Form: Lyophilized
      Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
      Applications: IHC,WB
      Application details: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse

      Western blot, 0.1-0.5μg/ml, Mouse
      Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2mg Na2HPO4, 0.01 mg NaN3.
    • 100 ug/vial / APC, Biotin, Cy3, FITC, Fluoro488, Fluoro550, Fluoro594, Fluoro647, PE
      Form: Liquid
      Storage: At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing.; At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
      Applications: WB,IHC,ELISA; Flow Cytometry
      Application details: Western blot, 0.25-0.5μg/ml
      Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml
      ELISA, 0.1-0.5μg/ml
      Flow Cytometry, 1-3μg/1x106 cellsContents: Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
    • 100 ug/vial / HRP
      Form: Liquid
      Storage: At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing.
      Applications: WB,IHC,ELISA
      Application details: Western blot, 0.25-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml
      ELISA, 0.1-0.5μg/ml
      Contents: Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4.
  • Lead time: typically ships in ~2-3 business days; timing may vary by selected option.
  • Storage: varies by selected option; see option details under Options.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target Cd33
Alternative names Myeloid cell surface antigen CD33;Sialic acid-binding Ig-like lectin 3;Siglec-3;CD33;Cd33;Siglec3;
UniProt # Q63994
Host Rabbit
Clonality
  • Polyclonal
Isotype
  • Rabbit IgG
Reactivity
  • Mouse
Applications
  • Immunohistochemistry
  • Western Blot
Immunogen E. coli-derived mouse CD33 recombinant protein (Position: D18-E240). Mouse CD33 shares 61.6% amino acid (aa) sequence identity with human CD33.
Molecular weight 44.8 kDa
Purification Immunogen affinity purified.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Cellular localization Cell membrane; Single-pass type I membrane protein.
Concentration Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Form Lyophilized
Storage Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Catalog no. (Mfr.) RP1105
Main SKU BHA21000871
Rabbit Polyclonal · Picoband®

Product Overview

This rabbit polyclonal antibody detects myeloid cell surface antigen CD33 in mouse samples and is validated for IHC and Western blot. The predicted molecular weight is 44.8 kDa, and the supplier reports an observed band at about 26 kDa.

CD33 (Siglec-3) is a sialic acid-binding immunoglobulin-like lectin expressed on myeloid cells and microglia. Through ITIM signaling it keeps immune cells in a resting state, and it is studied both as a target in acute myeloid leukemia and as a genetic risk factor in Alzheimer′s disease.

Target Myeloid cell surface antigen CD33 (gene Cd33; UniProt Q63994, mouse)
Host / Clonality / Isotype Rabbit / Polyclonal / Rabbit IgG
Reactivity Mouse
Form Lyophilized
Formulation Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.01 mg NaN3
Calculated MW 44.8 kDa
Observed MW 26 kDa
Storage As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles.

Validated Applications

IHC (paraffin sections) 0.5–1 µg/mL (Mouse)
Western blot 0.1–0.5 µg/mL (Mouse)

Samples with a confirmed band (WB): mosue lung extract (mouse spleen tissue.

Recommended loading (WB): 20–40 µg of total protein per lane.

Immunogen

Recombinant mouse CD33 fragment (Asp18–Glu240), expressed in E. coli. Sequence identity with the human ortholog: 61.6%.

Reactivity Notes

The supplier lists reactivity with mouse. UniProt places myeloid cell surface antigen CD33 in the cell membrane. Tissue expression noted by UniProt (mouse): Expressed on myeloid precursors in the bone marrow. The samples tested by the supplier (listed above) are a practical starting point for positive controls.

Safety

Handling: the formulation contains sodium azide as a preservative. Wear gloves, avoid contact with skin and eyes, and dispose of waste according to local regulations. For research use only; not for diagnostic or therapeutic procedures.
Q.Which applications is this antibody validated for, and at what dilution?
A.Validated applications with suggested starting dilutions: Western blot 0.1–0.5 µg/mL (Mouse); IHC (paraffin sections) 0.5–1 µg/mL (Mouse). Optimal dilutions should be determined by each laboratory.
Q.Which species does this antibody react with?
A.Validated reactivity: Mouse. Other species are not listed by the supplier.
Q.Has this antibody been tested on zebrafish samples?
A.Reactivity with zebrafish samples has not been tested by the supplier. Aligning the immunogen sequence with the zebrafish ortholog (e.g., by BLAST) helps estimate the chance of cross-reactivity.
Q.What band size should be expected in Western blot?
A.Reported by the supplier: calculated molecular weight 44.8 kDa; observed band at about 26 kDa. The observed band is much smaller than the calculated size, which can indicate a processed form or a shorter isoform; check the supplier images.
Q.What immunogen was used to raise this antibody?
A.Recombinant mouse CD33 fragment (Asp18–Glu240), expressed in E. coli. Sequence identity with the human ortholog: 61.6%.
Q.How should this antibody be stored?
A.As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

Shuli Man et al. (2016) Inhibition of lung cancer in diethylnitrosamine-induced mice by Rhizoma paridis saponins. Molecular Carcinogenesis. 10.1002/mc.22601

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