| Field | Specification |
|---|---|
| Target | |
| Alternative names | Myeloid cell surface antigen CD33;Sialic acid-binding Ig-like lectin 3;Siglec-3;CD33;Cd33;Siglec3; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived mouse CD33 recombinant protein (Position: D18-E240). Mouse CD33 shares 61.6% amino acid (aa) sequence identity with human CD33. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects myeloid cell surface antigen CD33 in mouse samples and is validated for IHC and Western blot. The predicted molecular weight is 44.8 kDa, and the supplier reports an observed band at about 26 kDa.
CD33 (Siglec-3) is a sialic acid-binding immunoglobulin-like lectin expressed on myeloid cells and microglia. Through ITIM signaling it keeps immune cells in a resting state, and it is studied both as a target in acute myeloid leukemia and as a genetic risk factor in Alzheimer′s disease.
| Target | Myeloid cell surface antigen CD33 (gene Cd33; UniProt Q63994, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.01 mg NaN3 |
| Calculated MW | 44.8 kDa |
| Observed MW | 26 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL (Mouse) |
|---|---|
| Western blot | 0.1–0.5 µg/mL (Mouse) |
Samples with a confirmed band (WB): mosue lung extract (mouse spleen tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Recombinant mouse CD33 fragment (Asp18–Glu240), expressed in E. coli. Sequence identity with the human ortholog: 61.6%.
Reactivity Notes
The supplier lists reactivity with mouse. UniProt places myeloid cell surface antigen CD33 in the cell membrane. Tissue expression noted by UniProt (mouse): Expressed on myeloid precursors in the bone marrow. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Shuli Man et al. (2016) Inhibition of lung cancer in diethylnitrosamine-induced mice by Rhizoma paridis saponins. Molecular Carcinogenesis. 10.1002/mc.22601