| Field | Specification |
|---|---|
| Target | |
| Alternative names | Hematopoietic progenitor cell antigen CD34;CD34;CD34; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human CD34 recombinant protein (Position: T151-L385). Human CD34 shares 79% amino acid (aa) sequence identity with mouse CD34. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects hematopoietic progenitor cell antigen CD34 in human and rat samples and is validated for Western blot, IHC, ICC/IF and ELISA. The predicted molecular weight is 40.7 kDa, and the supplier reports an observed band at about 105 kDa.
CD34 is a heavily glycosylated transmembrane sialomucin expressed on hematopoietic stem and progenitor cells and on vascular endothelium. It is thought to take part in cell adhesion and trafficking, and it is the standard marker used to identify and enrich hematopoietic stem and progenitor cells.
| Target | Hematopoietic progenitor cell antigen CD34 (gene CD34; UniProt P28906, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 40.7 kDa |
| Observed MW | 105 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human placenta tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human lymphoma tissue, human gastric cancer tissue, human glioma tissue, human ovarian cancer tissue, human placenta tissue, human liver cancer tissue, rat kidney tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): human colon cancer tissue, human placenta tissue.
Immunogen
Recombinant human CD34 fragment (Thr151–Leu385), expressed in E. coli. Sequence identity with the mouse ortholog: 79%.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places hematopoietic progenitor cell antigen CD34 in the membrane. Tissue expression noted by UniProt (human): Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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