| Field | Specification |
|---|---|
| Target | |
| Alternative names | Hematopoietic progenitor cell antigen CD34;CD34;CD34; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human CD34 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone BBH-3 is a rabbit monoclonal antibody that recognizes hematopoietic progenitor cell antigen CD34 in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 40.7 kDa, and the supplier reports an observed band at about 120 kDa.
CD34 is a heavily glycosylated transmembrane sialomucin expressed on hematopoietic stem and progenitor cells and on vascular endothelium. It is thought to take part in cell adhesion and trafficking, and it is the standard marker used to identify and enrich hematopoietic stem and progenitor cells.
| Target | Hematopoietic progenitor cell antigen CD34 (gene CD34; UniProt P28906, human) |
|---|---|
| Clone | BBH-3 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 40.7 kDa |
| Observed MW | 120 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): NIH/3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): human spleen tissue, human tonsil tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): HUVEC cells.
Immunogen
Synthetic peptide based on the sequence of human CD34.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places hematopoietic progenitor cell antigen CD34 in the membrane. Tissue expression noted by UniProt (human): Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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