| Field | Specification |
|---|---|
| Target | |
| Alternative names | Platelet glycoprotein 4; Fatty acid translocase; FAT; Glycoprotein IIIb; GPIIIB; Leukocyte differentiation antigen CD36; PAS IV; PAS-4; Platelet collagen receptor; Platelet glycoprotein IV; GPIV; Thrombospondin receptor; CD antigen CD36; CD36; GP3B; GP4 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human CD36 recombinant protein (Position: K233-N439). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets platelet glycoprotein 4 (gene CD36) in human, mouse and rat samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 53.1 kDa, and the supplier reports an observed band at about 88 kDa.
CD36 is a scavenger receptor that binds a wide range of ligands, including long-chain fatty acids, oxidized LDL, thrombospondin and collagen. It supports fatty acid uptake in muscle and adipose tissue, foam cell formation in macrophages and platelet responses, so it is studied in lipid metabolism, atherosclerosis and angiogenesis.
| Target | Platelet glycoprotein 4 (gene CD36; UniProt P16671, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 53.1 kDa |
| Observed MW | 88 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL (Human) |
Samples with a confirmed band (WB): human HL-60 cells, human HepG2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): HEL cells.
Immunogen
Recombinant human CD36 fragment (Lys233–Asn439), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places platelet glycoprotein 4 in the cell membrane, membrane raft, Golgi apparatus and apical cell membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yuan Zhang et al. (2022) Loss of CD226 protects apolipoprotein E-deficient mice from diet-induced atherosclerosis. Biochimica et Biophysica ACTA-Molecular Basis of Disease. 10.1016/j.bbadis.2022.166452
Runbin Sun et al. (2017) Orally Administered Berberine Modulates Hepatic Lipid Metabolism by Altering Microbial Bile Acid Metabolism and the Intestinal FXR Signaling Pathway. Molecular Pharmacology. 10.1124/mol.116.106617
Wu Xinmou et al. (2017) Effect of acute exposure to PFOA on mouse liver cells in vivo and in vitro. Environmental Science and Pollution Research. 10.1007/s11356-017-0072-5
