| Field | Specification |
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| Alternative Names | ADP-Ribosyl Cyclase 1, ADPRC 1, Cyclic ADP-Ribose Hydrolase 1, cADPR hydrolase 1, CADPR1 |
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| Target |
Overview
Anti-CD38 (extracellular) Antibody is an antibody targeting ADP-Ribosyl Cyclase 1, ADPRC 1, Cyclic ADP-Ribose Hydrolase 1, cADPR hydrolase 1, CADPR1 Polyclonal raised in Rabbit (Unconjugated). This antibody is commonly used in IFC, IHC, LCI, WB to detect, localize, or compare expression of the target across samples.
Key elements and design rationale
- Target: ADP-Ribosyl Cyclase 1, ADPRC 1, Cyclic ADP-Ribose Hydrolase 1, cADPR hydrolase 1, CADPR1 (also reported as ADP-Ribosyl Cyclase 1, ADPRC 1, Cyclic ADP-Ribose Hydrolase 1, cADPR hydrolase 1, CADPR1).
- Immunogen/epitope region: Extracellular, C-terminus..
- Homology note: Rat - identical The antibody will not recognize human CD38 (informative for cross-species interpretation).
- Species reactivity (as provided): Rat, Mouse.
- Lot quality control (as provided): Western blot analysis.
- Peptide confirmation: Confirmed by amino acid analysis and mass spectrometry.
- Blocking peptide: Available for antigen preadsorption control where appropriate.
- Conjugate/format: Unconjugated (may affect detection channel and background).
These attributes help researchers interpret whether signal reflects the intended target in a given assay and sample context.
Biological background
ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1, ADPRC1, also known as CD38, is a member of the ADP-ribosyl cyclase family. CD38 can act as an enzyme with NAD-depleting and intracellular signaling activity or as a receptor with adhesive functions.1,2CD38, composed of 300 amino acids, is a single chain glycoprotein with a single transmembrane segment that can behave topologically as either a type II or type III membrane protein depending on its orientation within the membrane. CD38 functions as an enzyme that utilizes nicotinamide adenine dinucleotide (NAD+) as a substrate and catalyzes the synthesis of nicotinamide (NAM) and ADPR.
Research relevance and current trends
- Profiling immune-marker expression across cell subsets with single-cell or flow-based readouts.
- Connecting receptor/ligand levels to activation state and cytokine programs.
- Applying genetic perturbation or orthogonal assays to support specificity and interpretation.
Common research applications
- Western blot (WB): compare target abundance/size across lysates and conditions; consider isoforms/PTMs.
- Immunohistochemistry (IHC): examine spatial distribution in tissue and relate signal to cell-type composition.
- Immunofluorescence/ICC: assess subcellular localization and co-localization with markers in cells or sections.
- Flow cytometry (direct/indirect): quantify target-positive populations and shifts in expression across subsets.
- Live cell imaging (LCI): support extracellular-epitope detection on non-permeabilized cells when appropriate.
Interpretation typically benefits from comparing matched sample sets (e.g., treated vs control, WT vs KO/KD) and using orthogonal readouts where feasible.
Notes for experimental interpretation
- Isoforms and post-translational modifications can shift apparent molecular weight or epitope accessibility across samples.
- Cross-species signal may depend on epitope conservation; consult the provided homology note when selecting models.
- Permeabilization, fixation, and antigen retrieval can change accessibility of intracellular vs extracellular epitopes.
- Conceptual control: antigen preadsorption (blocking peptide) can help assess signal dependence on the immunogen region.
- Provided control suggestions: Negative control: BLP-NT068.
- Application notes: see product-specific dilution/usage notes and control concepts provided in the dataset.
Application abbreviations: CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot. Species abbreviations: H- Human, M- Mouse, R- Rat.
Recommended controls: Blocking peptide: BLP-NT068; Negative control: BLP-NT068.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
