| Field | Specification |
|---|---|
| Target | |
| Gene symbol | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A BALB/c mouse was immunized with human peripheral lymphocytes. Fusion partner: P3-X63-Ag8.653. |
| Conjugate | |
| Purity | |
| Purification | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This monoclonal antibody (clone RIV9), raised in Mouse, recognizes CD3E. It is validated for ELISA, Flow Cytometry, Immunofluorescence, Immunohistochemistry.
Validated Applications
| Applications | ELISA, Flow Cytometry, Immunofluorescence, Immunohistochemistry |
|---|---|
| ELISA (solid phase | 0.1-100 µg/ml; tracer 0.001-100 µg/ml for 30 min at RT). Flow cytometry (0.5-1.0 µg/million cells in 0.1 ml). Immunofluorescence (0.5-1.0 µg/ml). Immunohistology (1.0-2.0 µg/ml for 30 min at RT; an antigen retrieval method for formalin-fixed tissues is unavailable to date). In vivo/in vitro stimulation (0.02-2.0 µg/ml). |
Optimal dilutions should be determined by the end user for each application and sample type.
Immunogen
A BALB/c mouse was immunized with human peripheral lymphocytes. Fusion partner: P3-X63-Ag8.653.
Specifications
| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Clone | RIV9 |
| Isotype | IgG3k |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Immunogen | A BALB/c mouse was immunized with human peripheral lymphocytes. Fusion partner: P3-X63-Ag8.653. |
| Purification | Affinity purified from tissue culture. |
| Purity | The antibody is purified from tissue culture and contains no host Ig. |
| Concentration | Can be provided as 100 µg/mL, 500 µg/mL or 1mg/mL. |
| Formulation | PBS with 0.02% Sodium Azide. |
| UniProt # | P07766 |
| Storage | Store for up to 1 year at 2-8°C upon receipt. |
Reactivity Notes
Validated reactivity: Human, Mouse, Rat. For other species, epitope conservation may allow cross-reactivity; confirm with appropriate controls.
Safety & Handling
This product is for research use only (RUO) and is not intended for diagnostic or therapeutic use. The formulation contains sodium azide; handle with standard laboratory precautions.
Related Products
Explore related antibodies and reagents for CD3E research from St John's Laboratory Ltd, or contact us for alternative formats.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
