| Field | Specification |
|---|---|
| Target | |
| Alternative names | T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3; W3/25 antigen; CD antigen CD4; Cd4 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived rat Cd4 recombinant protein (Position: K28-A246). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes T-cell surface glycoprotein CD4 in rat samples and is validated for IHC and ELISA. The predicted molecular weight is 51.4 kDa.
CD4 is a transmembrane glycoprotein on helper T cells, and also on some monocytes and macrophages, that acts as a co-receptor for MHC class II–peptide complexes. It recruits the kinase LCK to boost T-cell receptor signaling, and it is the primary receptor used by HIV-1 to enter cells.
| Target | T-cell surface glycoprotein CD4 (gene Cd4; UniProt P05540, rat) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 51.4 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 2–5 µg/mL (Rat) |
|---|---|
| ELISA | 0.1–0.5 µg/mL (Rat) |
Samples with confirmed staining (IHC): rat spleen tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant rat Cd4 fragment (Lys28–Ala246), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with rat. UniProt places T-cell surface glycoprotein CD4 in the cell membrane.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Xiaojie Wang et al. (2023) Treating cutaneous squamous cell carcinoma using 5-aminolevulinic acid polylactic-co-glycolic acid nanoparticle-mediated photodynamic therapy in a mouse model. International Journal of Nanomedicine. 10.2147/IJN.S71245
Yanrong Wang et al. (2007) Endocrine function and lymphocyte infiltration of newborn rat ovaries after ultrarapid freezing and allotransplantation. Fertility and Sterility. 10.1016/j.fertnstert.2006.11.063
Na Zhao et al. (2013) Interleukin-15 gene therapy and the mammalian target of rapamycin inhibitor everolimus inhibit the growth of metastatic breast cancer. Journal of Gene Medicine. 10.1002/jgm.2739
Xiong Hai-bo et al. (2005) Induction of immune tolerance with heart-thymus composite allotransplantation in rats. Journal of Central South University of Technology. 10.1007/s11771-005-0424-z
