| Field | Specification |
|---|---|
| Target | |
| Alternative names | Integrin alpha-IIb;GPalpha IIb;GPIIb;Platelet membrane glycoprotein IIb;CD41;Integrin alpha-IIb heavy chain;Integrin alpha-IIb light chain, form 1;Integrin alpha-IIb light chain, form 2;ITGA2B;GP2B, ITGAB; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human ITGA2B, different from the related mouse sequence by four amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects integrin alpha-IIb (gene ITGA2B) in human, mouse and rat samples and is validated for IHC and Western blot. The predicted molecular weight is 113.4 kDa, and the supplier reports an observed band at about 120–135 kDa.
Integrin alpha-IIb (ITGA2B, CD41) pairs with integrin beta-3 to form the platelet fibrinogen receptor αIIbβ3 (GPIIb/IIIa). When platelets are activated, the integrin shifts to a high-affinity state and binds fibrinogen and von Willebrand factor, linking platelets into aggregates. Defects cause Glanzmann thrombasthenia, and CD41 is a standard platelet and megakaryocyte marker.
| Target | Integrin alpha-IIb (gene ITGA2B; UniProt P08514, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 113.4 kDa |
| Observed MW | 120–135 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Mouse, Rat) |
Samples with a confirmed band (WB): human HEL cells, human K562 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse lung tissue, rat lung tissue, human mammary cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide from the C-terminal region of human ITGA2B. It differs from the mouse sequence at four residues.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places integrin alpha-IIb in the cell membrane. Tissue expression noted by UniProt (human): Isoform 1 and isoform 2 are expressed in platelets and megakaryocytes, but not in reticulocytes. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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Shan Hua et al. (2026) Enhanced Auricular Cartilage Regeneration via 3D-Printed Hydrogel With miR-92a-3p-Enriched Platelet-Rich Plasma-Derived Extracellular Vesicles. Advanced Healthcare Materials. 10.1002/adhm.202505389
Linzhong Yang et al. (2025) Bionic nanovesicles sequentially treat flaps with different durations of ischemia by thrombolysis and prevention of ischemia-reperfusion injury. Materials Today Bio. 10.1016/j.mtbio.2025.101529
Jinchao Yu et al. (2017) Thrombopoietic stimulating activity of rhTyrRS (Y341A). Scientific Reports. 10.1038/s41598-017-12073-4
Hongbo Fang et al. (2022) Blocking protease-activated receptor 4 alleviates liver injury induced by brain death. Biochemical and Biophysical Research Communications. 10.1016/j.bbrc.2022.01.074
Runfeng Ni et al. (2022) A mouse model of irradiation and spleen-thymus lymphocyte infusion induced aplastic anemia. Hematology. 10.1080/16078454.2022.2113356
Stephanie Arnold et al. (2022) Single and Multiplex Immunohistochemistry to Detect Platelets and Neutrophils in Rat and Porcine Tissues. Methods and Protocols. 10.3390/mps5050071
