| Field | Specification |
|---|---|
| Target | |
| Alternative names | Leukosialin;Galactoglycoprotein;GALGP;Leukocyte sialoglycoprotein;Sialophorin;CD43;SPN;CD43; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human CD43 recombinant protein (Position: A272-P400). Human CD43 shares 72% and 73% amino acid (aa) sequences identity with mouse and rat CD43, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects leukosialin (gene SPN) in human, mouse and rat samples and is validated for flow cytometry, ICC/IF, IHC and Western blot. The predicted molecular weight is 40.3 kDa, and the supplier reports an observed band at about 100–120 kDa.
Leukosialin (SPN, also known as CD43) is a heavily sialylated transmembrane glycoprotein and a major surface molecule on most leukocytes. It regulates T-cell activation, differentiation and migration, in part through association with ERM proteins, and CD43 is commonly used as a leukocyte marker in immunology and hematopathology.
| Target | Leukosialin (gene SPN; UniProt P16150, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 40.3 kDa |
| Observed MW | 100–120 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|---|
| IHC (frozen sections) | 0.5–1 µg/mL (Human) |
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Mouse, Rat) |
| Immunocytochemistry / Immunofluorescence | 0.5–1 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | 2 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human Jurkat cells, human K562 cells, human U937 cells, human CCRF-CEM cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse spleen tissue, rat spleen tissue, human tonsil tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Samples with confirmed staining (ICC/IF): K562 cells.
Samples with confirmed staining (ICC/IF): human tonsil tissue.
Samples with a confirmed signal (flow cytometry): P3NSI cells, BRL cells, HL-60 cells.
Immunogen
Recombinant human CD43 fragment (Ala272–Pro400), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 72% and 73%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places leukosialin in the membrane and cell projection. Tissue expression noted by UniProt (human): Cell surface of thymocytes, T-lymphocytes, neutrophils, plasma cells and myelomas. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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