| Field | Specification |
|---|---|
| Target | |
| Alternative names | CD44 antigen; CDw44; Epican; Extracellular matrix receptor III; ECMR-III; GP90 lymphocyte homing/adhesion receptor; HUTCH-I; Heparan sulfate proteoglycan; Hermes antigen; Hyaluronate receptor; Phagocytic glycoprotein 1; PGP-1; Phagocytic glycoprotein I; PGP-I; CD antigen CD44; CD44; LHR; MDU2; MDU3; MIC4 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human CD44 recombinant protein (Position: Q21-E518). Human CD44 shares 66.5% |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes CD44 antigen in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, flow cytometry and ELISA.
CD44 is a cell surface glycoprotein and the main receptor for hyaluronan. It mediates cell–cell and cell–matrix adhesion, lymphocyte homing and migration, and it is used as a marker of activated and memory T cells as well as a marker in cancer stem cell research.
| Target | CD44 antigen (gene CD44; UniProt P16070, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human, Mouse, Rat) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human CD44 fragment (Gln21–Glu518), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes CD44 antigen as secreted and also located in the cell membrane and cell projection. Tissue expression noted by UniProt (human): Detected in fibroblasts and urine (at protein level).
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Shengsheng Li et al. (2026) LncRNA WFDC21P mediates the tobacco carcinogen induced malignant transformation of human normal lung epithelial cells by regulating tumor stemness. Food and Chemical Toxicology. 10.1016/j.fct.2026.116116
Qian Chen et al. (2025) Genipin stabilized fibrin microbeads: Carrying cytokines to form niches for stem cell differentiation. Journal of Biomaterials Applications. 10.1177/08853282251344394
Kirsan-Kesici Cemre Buse et al. (2026) Genotype-dependent metabolic and inflammatory regulation at the ANRIL locus in human primary macrophages. Molecular Genetics and Genomics. 10.1007/s00438-026-02454-8
Xinyang Nie et al. (2024) MiR-21-5p Modulates Cisplatin-Resistance of CD44+ Gastric Cancer Stem Cells Through Regulating the TGF-β2/SMAD Signaling Pathway. International Journal of General Medicine. 10.2147/IJGM.S476647
XD Zhi et al. (2013) Schwann cells promote neuronal differentiation of bone marrow stromal cells. African Journal of Biotechnology. 10.5897/AJB10.1824
