| Field | Specification |
|---|---|
| Target | |
| Alternative names | CD44 antigen;CDw44;Epican;Extracellular matrix receptor III;ECMR-III;GP90 lymphocyte homing/adhesion receptor;HUTCH-I;Heparan sulfate proteoglycan;Hermes antigen;Hyaluronate receptor;Phagocytic glycoprotein 1;PGP-1;Phagocytic glycoprotein I;PGP-I;CD44;CD44;LHR, MDU2, MDU3, MIC4; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human CD44 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 28C33 is a rabbit monoclonal antibody that recognizes CD44 antigen in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, flow cytometry and immunoprecipitation. The predicted molecular weight is 81.5 kDa, and the supplier reports an observed band at about 82 kDa.
CD44 is a cell surface glycoprotein and the main receptor for hyaluronan. It mediates cell–cell and cell–matrix adhesion, lymphocyte homing and migration, and it is used as a marker of activated and memory T cells as well as a marker in cancer stem cell research.
| Target | CD44 antigen (gene CD44; UniProt P16070, human) |
|---|---|
| Clone | 28C33 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 81.5 kDa |
| Observed MW | 82 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): human U-87 MG cells, human HeLa cells, human PANC-1 cells, rat spleen tissue, rat thymus tissue, mouse spleen tissue, mouse thymus tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human CD44.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes CD44 antigen as secreted and also located in the cell membrane and cell projection. Tissue expression noted by UniProt (human): Detected in fibroblasts and urine (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Liang Dong-Min et al. (2024) m6A-methylated KCTD21-AS1 regulates macrophage phagocytosis through CD47 and cell autophagy through TIPR. Communications Biology. 10.1038/s42003-024-05854-x
Yujuan Zhang et al. (2022) MiR-31 improves spinal cord injury in mice by promoting the migration of bone marrow mesenchymal stem cells. PLoS One. 10.1371/journal.pone.0272499
Hua Jia et al. (2019) Combination of BMSCs-laden acellular nerve xenografts transplantation and G-CSF administration promotes sciatic nerve regeneration. Synapse. 10.1002/syn.22093
Shuyuan Guo et al. (2020) Multiple Intravenous Injections of Valproic Acid-Induced Mesenchymal Stem Cell from Human-Induced Pluripotent Stem Cells Improved Cardiac Function in an Acute Myocardial Infarction Rat Model. Biomed Research International. 10.1155/2020/2863501
Xinyang Nie et al. (2024) MiR-21-5p Modulates Cisplatin-Resistance of CD44+ Gastric Cancer Stem Cells Through Regulating the TGF-β2/SMAD Signaling Pathway. International Journal of General Medicine. 10.2147/IJGM.S476647
Lin Jian et al. (2014) Folium Sennae protects against hydroxyl radical-induced DNA damage via antioxidant mechanism: an in vitro study. Botanical Studies. 10.1186/1999-3110-55-16
