| Field | Specification |
|---|---|
| Target | |
| Alternative names | Receptor-type tyrosine-protein phosphatase C; Leukocyte common antigen; L-CA; T200; CD antigen CD45; PTPRC; CD45 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human CD45, different from the related mouse sequence by eight amino acids, and from the related rat sequence by ten amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 2H3 is a mouse monoclonal antibody that recognizes receptor-type tyrosine-protein phosphatase C (gene PTPRC) in human samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 147.5 kDa, and the supplier reports an observed band at about 220 kDa.
CD45 (PTPRC) is a transmembrane tyrosine phosphatase present on all nucleated blood cells. By removing inhibitory phosphates from Src family kinases such as LCK and FYN it enables T- and B-cell antigen receptor signaling, and its isoforms (for example CD45RA and CD45RO) are widely used to mark leukocyte subsets.
| Target | Receptor-type tyrosine-protein phosphatase C (gene PTPRC; UniProt P08575, human) |
|---|---|
| Clone | 2H3 |
| Host / Clonality / Isotype | Mouse / Monoclonal / Mouse IgG2b |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 147.5 kDa |
| Observed MW | 220 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human Jurkat cells, human Raji cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): human lung cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): human tonsli tissue.
Samples with a confirmed signal (flow cytometry): Raji cells.
Immunogen
Synthetic peptide from the C-terminal region of human CD45. It differs from the mouse sequence at eight residues and from the rat sequence at ten residues.
Reactivity Notes
The supplier lists reactivity with human. UniProt places receptor-type tyrosine-protein phosphatase C in the cell membrane, membrane raft and synapse. Tissue expression noted by UniProt (human): Isoform 1: Detected in thymocytes. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
