| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Aldehyde dehydrogenase, mitochondrial; ALDH class 2; ALDH-E2; ALDHI; ALDH2; ALDM |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human CD45, different from the related mouse sequence by eight amino acids, and from the related rat sequence by ten amino acids. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-CD45 Antibody Picoband® (monoclonal, 2H3) is an antibody for PTPRC detection raised in Mouse (Monoclonal, clone Clone: 2H3, Mouse IgG2b), with reported reactivity: Human. Commonly used in WB, IHC, IF, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: PTPRC (aldehyde dehydrogenase 2 family member); UniProt: P08575
- Antibody format: Mouse, Monoclonal, clone Clone: 2H3, Mouse IgG2b
- Molecular weight: 220 kDa
- Applications: WB, IHC, IF, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-CD45 Antibody Picoband® (monoclonal, 2H3) catalog # M00555-5.
Biological background
Biological context: Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification.
Expression and localization notes: cellular localization: Mitochondrion matrix, tissue context: Plasma, liver, kidney, heart, brain, small intestine and lung. In the plasma, associated with HDL..
Common research applications
- Western blotting (WB): Compare PTPRC levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of PTPRC in tissue sections, considering fixation and antigen retrieval effects.
- Immunofluorescence / ICC: Assess subcellular localization patterns and co-localization with compartment markers in cultured cells.
- Flow cytometry: Quantify PTPRC-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: CD45 (Cluster of Differentiation 45), also known as PTPRC, LCA or CD45R, is an enzyme that, in humans, is encoded by the PTPRC gene. It is a member of the protein tyrosine phosphatase (PTP) family. CD45 is a major high molecular mass leukocyte cell surface molecule which is also an integral membrane protein tyrosine phosphatase. The cytogenetic location of CD45 is 1q31.3-q32.1. This gene is especially a prototype for transmembrane protein-tyrosine phosphatase (PTP). Targeted disruption of the CD45 gene leads to enhanced cytokine and interferon receptor-mediated activation of JAKs and STAT proteins. In vitro, CD45 ly dephosphorylates and binds to JAKs. Functionally, CD45 negatively regulates interleukin-3-mediated cellular proliferation, erythropoietin-dependent hematopoiesis, and antiviral responses in vitro and in vivo. In addition, CD45 has been best studied in T cells, where it determines T cell receptor signaling thresholds. CD45 is moved into or out of the immunological synapse (IS) membrane microdomain depending on the relative influence of interaction with the extracellular galectin lattice or the intracellular actin cytoskeleton. Galectin interaction can be finetuned by varying usage of the heavily Oglycosylated spliced regions and sialylation of Nlinked carbohydrates.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Mitochondrion matrix
- Tissue details: Plasma, liver, kidney, heart, brain, small intestine and lung. In the plasma, associated with HDL.
- Research category: Atherosclerosis,Cancer,Cardiovascular,Cell Biology,Diabetes,Diabetes-associated,Drug Metabolism,Heart Disease,Lipid and Lipoprotein Metabolism,Lipid Metabolism,Lipids/Lipoproteins,Metabolic Signaling Pathways,Metabolism,Neurodegenerative Disease,Neurology Process,Neuroscience,Oxidative Stress,Pathways and Processes,Redox Metabolism,Signal Transduction
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
