| Field | Specification |
|---|---|
| Target | |
| Alternative names | Leukocyte surface antigen CD47;Antigenic surface determinant protein OA3;Integrin-associated protein;IAP;Protein MER6;CD47;CD47;MER6; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human CD47 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone D-3 is a rabbit monoclonal antibody that recognizes leukocyte surface antigen CD47 in human, mouse and rat samples and is validated for Western blot, ICC/IF and flow cytometry. The predicted molecular weight is 35.2 kDa, and the supplier reports an observed band at about 54 kDa.
CD47 is a widely expressed immunoglobulin superfamily membrane protein that binds SIRPα on macrophages to deliver a "don′t eat me" signal, and it also serves as a receptor for thrombospondin-1. It regulates phagocytosis, cell migration and integrin signaling, and it is a major focus of innate immune checkpoint research in cancer.
| Target | Leukocyte surface antigen CD47 (gene CD47; UniProt Q08722, human) |
|---|---|
| Clone | D-3 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 35.2 kDa |
| Observed MW | 54 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:1000–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:50 |
Immunogen
Synthetic peptide based on the sequence of human CD47.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places leukocyte surface antigen CD47 in the cell membrane. Tissue expression noted by UniProt (human): Very broadly distributed on normal adult tissues, as well as ovarian tumors, being especially abundant in some epithelia and the brain.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Li Yin et al. (2017) Expressions Profiles of the Proteins Associated with Carbohydrate Metabolism in Rat Liver Regeneration. Biomed Research International. 10.1155/2017/8428926
