| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | L-selectin;CD62 antigen-like family member L;Leukocyte adhesion molecule 1;LAM-1;Leukocyte surface antigen Leu-8;Leukocyte-endothelial cell adhesion molecule 1;LECAM1;Lymph node homing receptor;TQ1;gp90-MEL;CD62L;SELL;LNHR, LYAM1; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E. coli-derived human CD62L recombinant protein (Position: W39-K151). Human CD62L shares 82.3% and 80.5% amino acid (aa) sequence identity with mouse and rat CD62L, respectively. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of SELL in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-CD62L/SELL Antibody Picoband® catalog # A00652. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E. coli-derived human CD62L recombinant protein (Position: W39-K151). Human CD62L shares 82.3% and 80.5% amino acid (aa) sequence identity with mouse and rat CD62L, respectively. (reported region: W39-K151).
- Molecular weight context: reported MW: 90 kDa; calculated MW: 42187 MW
- Reactivity: Human
- Applications: ELISA, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
L-selectin. CD62L, also known as L-selectin, is a cell adhesion molecule found on leukocytes. It belongs to the selectin family of proteins, which recognize sialylated carbohydrate groups. It is cleaved by ADAM17. SELL (L-selectin) is a cell surface component that is a member of a family of adhesion/homing receptors which play important roles in leukocyte-endothelial cell interactions. The molecule is composed of multiple domains: one homologous to lectins, one to epidermal growth factor, and two to the consensus repeat units found in C3/C4 binding proteins. L-selectin acts as a homing receptor for leukocytes to enter secondary lymphoid tissues via high endothelial venules. Ligands present on endothelial cells will bind to leukocyte expressing L-selectin, slowing leukocyte trafficking through the blood, and facilitating entry into a secondary lymphoid organ at that point. Functional note: Cell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia. . Reported localization: Membrane; Single-pass type I membrane protein. Expression/tissue context: Expressed in B-cell lines and T-lymphocytes. .
Research relevance and current trends
- Adaptive Immunity: Researchers commonly examine how SELL relates to this theme using model systems and orthogonal readouts.
- Cardiovascular: Researchers commonly examine how SELL relates to this theme using model systems and orthogonal readouts.
- Hematopoietic Progenitors: Researchers commonly examine how SELL relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative SELL levels across conditions; band patterns may reflect isoforms and processing.
- ELISA-compatible use: when applicable, interpret signal as relative abundance across sample sets with consistent handling and dilution strategy.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
